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This detailed volume explores the latest methods that can be used
to probe mRNA decay pathways and identify mRNA-binding protein
targets as well as miRNA targets. Subjects include metabolic
labelling and RNAseq methods for determining RNA decay rates,
approaches for discovering RNA-binding protein targets,
bioinformatics, miRNA targets and novel components of the
miRNA-directed decay pathway, and recently developed approaches for
studying nonsense-mediated mRNA decay, among other areas. Written
for the highly popular Methods in Molecular Biology series,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, mRNA Decay:
Methods and Protocols serves as an ideal guide for molecular
biologists, geneticists, and developmental biologists with an
interest in understanding how normal development and tissue
homeostasis is regulated and how these processes are perturbed in
inherited and acquired diseases.
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