This volume explores the latest techniques used to study neurodevelopmental diseases (NDD) that range from molecular aspects to integrated research approaches and brain imaging in living rodents. Chapters in this book cover topics such as protocols to deliver shRNA in vitro and in vivo using lentiviral particles to knock-down specific protein expression; experimental procedures to use recombinant fluorescent probes to visualize endogenous proteins at the mammalian synapse; CRISPR/Cas9 toolkit to assess either gain- or loss-of-gene function in brain organoids; detailed protocols to use in vivo manipulations to correct the behavioral phenotypes in cognitive disorder mouse lines; and experimental approaches to genetically engineer macaque models of NDDs and investigate how genetic predisposition may cause neural and functional alterations. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Translational Research Methods in Neurodevelopmental Disorders is a valuable resource for all researchers who want to learn more about this important and developing field.

The "juvenile operetta" was a theatrical artform that blossomed from 1900 to 1950, and in this overview, we examine over 100 examples in all their weird and wonderful glory. With stories that range from princesses in distress to Canadians in deepest Africa to Chinese businessmen coping with "The New Woman," they are not only giddy delights onto themselves but an overview of American social structure during the days surrounding the Great Depression. An invaluable addition to any theatre history library, NO ONE EVER SAID will provide hours of shock and delight.

A series of three one-act plays - a young man encountering sex for the first time, a verging-on-middle-age man meeting Death for volleyball and beer, and the two incredibly ancient gay men who find themselves props in a university lecture. Three moments in life that "could" be serious, but turn out to be... very different indeed...

Wikileaks, gay marriage, and the perils of cleaning the fridge... it's just another day at "Doc and Raider," one of the world's longest running LGBT comic strips. Follow the adventures of these two Canadian gay boys trapped in a world definitely not of their own design, as they tapdance around the cultural, political, and societal minefields of White Parties, Gay Evolution, and Proper Canadian MMA. In this, the first collection of the online version, everything from illegal aliens to the potential perils of three-ways gets tossed in the mix for your reading pleasure.

This fifth collection of "Doc and Raider" strips, from 2011, takes the boys into "straight camp," a glimpse at what's written on the back of the Constitution, and a test to see if they're "fairy enough." And just what is it about Doc's Mummy? Find out in the most Googled strip of that year: "Breaking Dawn, Part II..".

"Les citaines" (The Cityboys) are those urban gay men who would be lost without a skyline in view - and this, the first collection of Sean Martin's long running series, lets you into their world. At first glance, it's a simple one: dinner gets burned, music is rehearsed, a kiss is shared... but in each case, there is always a bit more lurking in the shadowy details. What is that story, you ask? That, dear reader, is what you bring to the table, which guarantees that LES CITAINS is a book you will return to again and again, to find new adventures in the minutae of urban gay life...

In this first collection of strips from 2012, the boys encounter 3D television, careers in the MMA, the mortgage crisis... not to mention the never-ending presidential debates. Jesus drops by a couple of times to give some insight into the original editors of the Bible's pre-pubication manuscript, and Doc has to deal with the horror of winning the lottery...

VOICE (a distant rumble) I'M LISTENING. YOU HAVE ONE MINUTE. DORE. We need help. I'm trying to get back to Kansas - VOICE. AH... EXOTIC PORTS OF CALL. THOSE ARE ALWAYS GOOD. "RETURN TO KANSAS " NO, "KANSAS" -- No, wait "KANSAS " WITH AN EXCLAMATION MARK. IT MIGHT PLAY. IF IT DOES, I COULD ARRANGE A TOURING PARTY TO PLAY THE STICKS OF YOUR CHOICE. I GATHER KANSAS IS THE STICKS. DORE "(shrugs in reply)" Folks seem to think so.

In this wide-ranging collection of "Doc and Raider" cartoons from 2010, the boys give their take on everything from earthquakes in China to the BP Deepwater oil disaster to California's Prop 8. It's a wild ride, with possible issues of unexpected acceleration

CHIP. If I may, Captain. Starting with the Treaty of 4612, the Asu bound several worlds to an international coalition that would see the Asu in a position to leverage the upside return of ferrous substrates as described in such treaties, in effect putting the Asu in a position of coalescing economic variance factors into a single marketable stream, while at the same time imposing trade embargoes on world systems that failed to buy into such agreements. This, coupled with their well-documented usage percentages, as well as the ongoing treaty discussions of the DRATL in the Rovenoe System, has led to the Asu's re-positioning themselves at the top of the economic (and quite possibly governmental) hierarchy while at the same time maintaining a firm and well entrenched grasp on much needed commodities. A rather remarkable system, I must say. SKIP "(his eyes dazed)" Huh? JOE. Get to it, Chip: what do they want? CHIP "(as though it's perfectly obvious)" Iron. JOE "(with a snort)"Right. SKIP. As if, Robotguy. Iron is probably the most available element out there next to hydrogen. "(with a snicker)" You might as well say they want oil or something.

When an English-speaking leatherman from the US and a French-speaking Catholic priest from Canada fall in love, you can expect more than a few bumps on the road to Happily Ever After. But when it's Raider's best friend Elliot and Doc's twin brother Gilles, nothing - language, distance, or even a storage bin filled with empty boxes - can get in their way. Is there a happy ending? Mais oui, m'sieur