Sixty years ago at the Waite Agricultural Research Institute, G. Samuel, a plant pathologist, and C. S. Piper, a chemist, published their conclusion that the cause of roadside take-all, a disease of oats, was manganese deficiency. This report, together with the concurrent and independent studies of W. M. Carne in Western Australia were the first records of manganese deficiency in Australia and came only six years after McHargue's paper which is generally accepted as the final proof of the essentiality of this element. There must have been a few doubts for some people at the time, however, as the CAB publication, 'The Minor Elements of the Soil' (1940) expressed the view that further evidence to this effect was provided by Samuel and Piper. Their historic contributions are recognised by the International Symposium on Manganese in Soils and Plants as it meets on the site of their early labours to celebrate the 60th anniversary. This year Australians also acknowledge 200 years of European settlement in this country and so the Symposium is both a Bicentennial and a diamond jubilee event which recognises the impact of trace elements on agricultural development in Australia. In a broader sense, a symposium such as this celebrates, as it reviews, the efforts of all who over the ages have contributed to our knowledge of manganese in soils and plants.

Increased atmospheric nitrogen deposition and changes in the management of heathlands have caused a significant change in the species composition of the NW-European heathlands. This change from ericaceous towards gramineous dominance is analysed in detail in Heathlands: Patterns and Processes in a Changing Environment. Special emphasis is put on the effects of increased atmospheric nutrient input on nutrient cycling, competition between plant species and plant--herbivore interactions. The possibilities for the long-term conservation of heathlands are discussed by considering regeneration from seed and the usefulness of mathematical management models. The book provides a synthesis of pure and applied plant ecology. It is vital reading for plant ecologists, biological conservationists, heathland managers and government decision makers.

As drought tolerance is a multidirnensional stress, drought tolerance study is a multidisciplinary adventure. In 1992, the network INTERDROUGHT was created with the objective of joining the scientists of the different fields of research involved in drought tolerance study. The network was funded by the EEC and gathered 25 European teams specialized in molecular biology, physiology and geneties. 1\vo workshops were successively organized in 1993 in Sitges (Spain) and in 1994 in Ischia (Italy). After those two European workshops, the necessity of opening the network to the whole scientific community was already clear, and in 1995 the first INTERDROUGHT international conference was held in Montpellier (France). During this meeting, eleven speakers were invited to present a review in their field of research, in a way accessible to all researchers and students, especially those who are not familiar wlth one of the three fields of interest. These eleven reviews are presented in this book. From these reviews three major difficulties arose for drought tolerance irnprovement: - the definition of the drought stress that plants experience; -the differentiation between non adaptive and adaptive response to drought stress; -the identification of the adaptive responses that improved drought yield without decreasing significantly the potential yield. The use of integrated strategies of research will certainly provide irnportant results, such as the recent data obtained on molecular and physiologieal analysis of Arabidopsis mutants.

With one volume published each year, this series keeps scientists and students current with the latest developments and results in all areas of the plant sciences. This present volume includes insightful reviews covering genetics, cell biology, physiology, comparative morphology, systematics, ecology, and vegetation science.

Lipids in Photosynthesis provides readers with a comprehensive view of the structure, function and genetics of lipids in plants, algae and bacteria, with special emphasis on the photosynthetic apparatus in thylakoid membranes. This volume includes the historical background of the field, as well as a full review of our current understanding of the structure and molecular organization of lipids and their role in the functions of photosynthetic membranes. The physical properties of membrane lipids in thylakoid membranes and their relationship to photosynthesis are also discussed. Other topics include the biosynthesis of glycerolipids and triglycerides; reconstitution of photosynthetic structures and activities with lipids; lipid-protein interactions in the import of proteins into chloroplasts; the development of thylakoid membranes as it relates to lipids; genetic engineering of the unsaturation of membrane glycerolipids, with a focus on the ability of the photosynthetic machinery to tolerate temperature stress; and the involvement of chloroplast lipids in the reactions of plants upon exposure to stress. This book is intended for a wide audience and should be of interest to advanced undergraduate and graduate students and to researchers active in the field, as well as to those scientists whose fields of specialization include the biochemistry, physiology, molecular biology, biophysics and biotechnology of membranes.

Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.

The contributions are written by leading scientists in their field. The book is divided into 5 sections on Imaging, Structure, Optical and laser spectroscopy, Magnetic resonance and on Theory, respectively. Each chapter describes the basic concepts of the technique, practical applications and some of the scientific results. Possibilities and limitations from a technical as well as a scientific point of view are addressed, allowing the reader not only to recognize the potential of a particular method for his/her own quest, but to assess the resources that are required for implementation.

The primary aim of Wood Structure and Environment is to reveal the hidden ecological richness in stems and roots from trees, shrubs and herbs. The detailed, lucid text will inspire researchers to consider the anatomic microcosm of wood plants and use it as a retrospective source of information, solving problems related to ecophysiology, competition, site conditions, population biology, earth science, wood quality and even human history.

Plant neurobiology is a newly emerging field of plant sciences. It covers signalling and communication at all levels of biological organization - from molecules up to ecological communities. In this book, plants are presented as intelligent and social organisms with complex forms of communication and information processing.

Authors from diverse backgrounds such as molecular and cellular biology, electrophysiology, as well as ecology treat the most important aspects of plant communication, including the plant immune system, abilities of plants to recognize self, signal transduction, receptors, plant neurotransmitters and plant neurophysiology. Further, plants are able to recognize the identity of herbivores and organize the defence responses accordingly. The similarities in animal and plant neuronal/immune systems are discussed too. All these hidden aspects of plant life and behaviour will stimulate further intense investigations in order to understand the communicative plants in their whole complexity.

Recent years have brought an upsurge of interest in the study of arbuscular mycorrhizal (AM) fungi, partly due to the realization that the effective utilization of these symbiotic soil fungi is likely to be essential in sustainable agriculture. Impressive progress has been made during the last decade in the study of this symbiosis largely as a result of increasing exploitation of molecular tools. Although early emphasis was placed on the use of molecular tools to study physiological processes triggered by the symbiosis, such as expression of symbiosis-specific polypeptides and modulation of host defences, other applications await. It was obvious to us that gathering leaders in the field to summarize these topics and point out research needs was necessary if we were to understand the physiology and function of AM fungi at a molecular level. In addition, we have taken the opportunity to present these reviews in a logical sequence of topics ranging from the initiation of the life cycle of the fungus to its functions in plant growth and in the below ground ecosystem. It was a challenge to limit this flood of information to the confines of one text. This is a very exciting time for mycorrhiza biologists and it is our hope that some of this excitement is conveyed to our readers.

The Second International Congress on Photosynthesis Research took place in Stresa, Italy during June 24-29, 1971; two centuries after the discovery of Photosynthesis by Joseph Priestley in 1971. This important anniversary was celebrated at the Congress by a learned account of Priestley's life and fundamental discoveries given by Professor Robin HILL, F. R. S. Professor HILL's lecture opens the first of the three volumes which contains the contributions presented at the Congress. The manuscripts have been distributed into three volumes. Volume I con tains contributions in the areas of primary reactions and electron transport; Volume II ion transport and photophosphorylation, and Volume III carbon assimilation, regulatory phenomena, developmental aspects, and from the two special sessions of the Congress devoted to evolution and photorespiration. It is realized that this division is necessarily somewhat arbitrary since many contributions relate to more than one of the above mentioned titles. However, the large number of contributions (over 3000 typed pages) made it impossible to publish the proceedings in less than three volumes. The printing of these volumes and the organization of the Congress were made possible by a contribution from the Consigio Nazionale delle Ricerche of Italy. The generous support of the Istituto Lombardo Acca demia di Scienze e Lettere to the publication of these proceedings is gratefully acknowledged. The editors wish to express their appreciation to all the scientists who contributed the results of the investigations, for their coopera tion; and to Drs."

Since the beginning of agricultural production, there has been a continuous effort to grow more and better quality food to feed ever increasing popula tions. Both improved cultural practices and improved crop plants have al lowed us to divert more human resources to non-agricultural activities while still increasing agricultural production. Malthusian population predictions continue to alarm agricultural researchers, especially plant breeders, to seek new technologies that will continue to allow us to produce more and better food by fewer people on less land. Both improvement of existing cultivars and development of new high-yielding cultivars are common goals for breeders of all crops. In vitro haploid production is among the new technologies that show great promise toward the goal of increasing crop yields by making similar germplasm available for many crops that was used to implement one of the greatest plant breeding success stories of this century, i. e., the development of hybrid maize by crosses of inbred lines. One of the main applications of anther culture has been to produce diploid homozygous pure lines in a single generation, thus saving many generations of backcrossing to reach homozygosity by traditional means or in crops where self-pollination is not possible. Because doubled haploids are equivalent to inbred lines, their value has been appreciated by plant breeders for decades. The search for natural haploids and methods to induce them has been ongoing since the beginning of the 20th century."

Adaptation and evolution of terrestrial plants depend, to a large extent, on their ability to acquire nutrients. This is a modern and integrative treatment of the mechanisms controlling plant nutrient uptake and how plants respond to changes in the environment. The following key topics are covered: soil nutrient bioavailability; root responses to variations in nutrient supply; nitrogen fixation; regulation of nutrient uptake by internal plant demand; root characteristics; kinetics of nutrient uptake; root architecture; life span; mycorrhizae; responses to climate change. This integrated view helps us to understand the mechanisms that govern present-day plant communities and is indispensable in models designed to predict the response of plants to a changing climate.

Flowering plants dominate much of the Earth's surface and yet, as sessile organisms, they must constantly resist attack by numerous voracious herbivores. Survival in the face of an abundance of insect predators relies on sophisticated resistance systems allowing plants to escape from herbivory in time or in space, to confront herbivores directly, or to fight them indirectly by remarkable collaborations with other species. Until recently, plant resistance was believed to be constitutive, i.e. ever-present and independent from herbivore attack. However, plants were discovered to respond actively to herbivory through the mobilization of specific defenses, and this discovery opened an exciting new field of research.
This book provides a thorough overview of the anatomical, chemical, and developmental features contributing to plant defense, with particular emphasis on plant responses that are induced by wounding or herbivore attack.

The Conference on Tropical Rainforest Research: Current Issues was organised by the University of Brunei Darussalam and The Royal Geographical Society, London, and held in Bandar Seri Begawan, Brunei Darussalam, over 8 days in April 1993. Over 160 participants from 22 countries attended the Conference, which was opened on the 9th April by the Brunei Darussalam Minister for Home Affairs, Yang Berhormat Pehin Orang Kaya Laila Setia Bakti Diraja Dato Laila Utama Haji Awang Isa bin Datu Perdana Menteri Dato Laila Utama Haji Awang Ibrahim. The conference was initially intended to provide a forum to present the results of the expedition into the lowland mixed dipterocarp forest of the Temburong District of Brunei Darussalam, which had been jointly organised by the University of Brunei Darussalam and The Royal Geographical Society, London. The 15-month expedition, lasting from January 1991 to March 1992, was based at the then newly-completed Kuala Belalong Field Studies Centre, a research and education facility set up by the University with funds provided by the Brunei Government and Brunei-Shell Petroleum Sdn Bhd. The expedition, with over 70 scientists taking part, received financial support from a wide range of sponsors and Corporate Patrons, including Royal Brunei Airlines, the Baring Foundation, Daiwa-Dicam, Greencard Trust, the Hongkong Bank, Morgan Grenfell and Nomura-Nimco. The conference itself was supported by donations from Brunei-Shell Sdn Bhd, Royal Brunei Airlines and Standard Chartered Bank.

In this comprehensive and stimulating text and reference, the authors have succeeded in combining experimental data with current hypotheses and theories to explain the complex physiological functions of plants. For every student, teacher and researcher in the plant sciences it offers a solid basis for an in-depth understanding of the entire subject area, underpinning up-to-date research in plant physiology. The authors vividly explain current research by references to experiments, they cite original literature in figures and tables, and, at the end of each chapter, list recent references that are relevant for a deeper analysis of the topic. In addition, an abundance of detailed and informative illustrations complement the text.

Plant secondary metabolism is an economically important source of fine chemicals, such as drugs, insecticides, dyes, flavours, and fragrances. Moreover, important traits of plants such as taste, flavour, smell, colour, or resistance against pests and diseases are also related to secondary metabolites. The genetic modification of plants is feasible nowadays. What does the possibility of engineering plant secondary metabolite pathways mean? In this book, firstly a general introduction is given on plant secondary metabolism, followed by an overview of the possible approaches that could be used to alter secondary metabolite pathways. In a series of chapters from various authorities in the field, an overview is given of the state of the art for important groups of secondary metabolites. No books have been published on this topic so far. This book will thus be a unique source of information for all those involved with plants as chemical factories of fine chemicals and those involved with the quality of food and ornamental plants. It will be useful in teaching graduate courses in the field of metabolic engineering in plants.

Proceedings of a Seminar in the CEC Programme of the Coordination of Research on Plant Protein Improvement, held in Gembloux, Belgium, Sept. 3-5, 1985

This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.

The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.

Progress in photosynthesis research is strongly dependent on instrumentation. It is therefore not surpr- ing that the impressive advances that have been made in recent decades are paralleled by equally impressive advances in sensitivity and sophistication of physical equipment and methods. This trend started already shortly after the war, in work by pioneers like Lou Duysens, the late Stacy French, Britton Chance, Horst Witt, George Feher and others, but it really gained momentum in the seventies and especially the eighties when pulsed lasers, pulsed EPR spectrometers and solid-state electronics acquired a more and more prominent role on the scene of scientific research. This book is different from most others because it focuses on the techniques rather than on the scientific questions involved. Its purpose is three-fold, and this purpose is reflected in each chapter: (i) to give the reader sufficient insight in the basic principles of a method to understand its applications (ii) to give information on the practical aspects of the method and (iii) to discuss some of the results obtained in photosynthesis research in order to provide insight in its potentalities. We hope that in this way the reader will obtain sufficient information for a critical assessment of the relevant literature, and, perhaps more important, will gain inspiration to tackle problems in his own field of research. The book is not intended to give a comprehensive review of photosynthesis, but nevertheless offers various views on the exciting developments that are going on.

Nitrogen is an essential element for plant growth. During the green revolution nitrogenfertilisation was responsible for spectacular yield increases. At present yield is balanced with commitments towards the environment and sustainable agriculture. For agro-biotechnology comprehensive knowledge of plant functioning is needed. Yield improvement and accumulation of essential nitrogen compounds is relying on selection and gene technologies.

Research on the uptake, acquisition and assimilation of nitrogen, as well as the synthesis and storage of reserve and defence N-compounds, therefore, is essential. The third volume in the Plant Ecophysiology series integrates functional and molecular physiology with ecophysiological and sustainable agricultural approaches to get a better understanding of the regulation and the impact of environmental and stress signals on nitrogen acquisition and assimilation. The book is of interest for advanced students and junior researchers and supplies comprehensive information for scientists working in the field of nitrogen metabolism and readers interested in sustainable development.

The aim of this book is to give an overview of the most important aspects of physiological and biochemical basis for metal toxicity and tolerance in plants. The book is expected to serve as a reference to university and college teachers, students of plant sciences, environmental biology, environmental biotechnology, agriculture, horticulture, forestry, plant molecular biology, and genetics.

A Personal Note I decided to initiate Orchid Biology: Reviews and Perspectives in about 1972 and (alone or with co-authors) started to write some of the chapters and the appendix for the volume in 1974 during a visit to the Bogor Botanical Gardens in Indonesia. Professor H. C. D. de Wit of Holland was also in Bogor at that time and when we discovered a joint interest in Rumphius he agreed to write a chapter about him. I visited Bangkok on my way home from Bogor and while there spent time with Professor Thavorn Vajrabhaya. He readily agreed to write a chapter. The rest of the chapters were solicited by mail and I had the complete manuscript on my desk in 1975. With that in hand I started to look for a publisher. Most of the publishers I contacted were not interested. Fortunately Mr James Twiggs, at that time editor of Cornell University Press, grew orchids and liked the idea. He decided to publish Orchid Biology: Reviews and Per spectives, and volume I saw the light of day in 1977. I did not know if there would be a volume II but collected manuscripts for it anyway. Fortunately volume I did well enough to justify a second book, and the series was born. It is still alive at present - 20 years, seven volumes and three publishers later. I was in the first third of my career when volume I was published."

Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry.

The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants.

This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.

Bioactive Carbohydrate Polymers is probably the first book dealing with the latest in the field of polysaccharides and related products and their biological activities, especially the immunological effects. The different chapters describe the structure and bioactivity of polysaccharides from plants used in traditional medicine in different parts of the world, especially China, Japan and Europe. The focus of the book is on immunologically active plant and seaweed polysaccharides, pharmacological activities of sulphated polysaccharides of animal and seaweed origin, and on possible activities of polysaccharides in our food. Methods for isolation and characterisation of the polymers with chemical and enzymatic methods is covered, as well as discussions on the different biological test-systems and the information they provide. This book will be useful to scientists and postgraduate students working with polysaccharides and their possible uses, and should be of interest for people working in the areas of chemistry, biology, pharmacy and medicine.

Wheat breeders have achieved significant results over the last fifty years in research on mankind's one of the most important crops. Classical genetic and breeding methods, far broader international cooperation than was experienced in earlier periods, and improvements in agronomic techniques have led to previously unimaginable development in the utilisation of wheat for human consumption. The contribution of wheat researchers is particularly noteworthy since these results have been achieved at a time when the world population has grown extremely dynamically. Despite this demographic explosion, of a proportion never previously experienced, thousands of millions of people have been saved from starvation, thus avoiding unpredictable social consequences and situations irreconcilable with human dignity. Despite these developments in many regions of the world food supplies are still uncertain and the increase in the world's wheat production has not kept pace with the population increase during the last decade. Due to the evils of civilisation and the pollution of the environment there is a constant decline in the per capita area of land suitable for agricultural production. Based on population estimates for 2030, the present wheat yield of around 600 million tonnes will have to be increased to almost 1000 million tonnes if food supplies are to be maintained at the present level.