This book was originallyconceived in the form ofa second edition
ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies
in Biology' series and entitled Genetic Engineering - Cloning
DNA.It very rapidly became apparent that with the impact
ofrecombinant DNA techniques being feIt in so many areas ofblology,
it was going to be difficultifnotimpossible to keepthe bookwithin
the space confines of these little monographs. The stays were
therefore loosened and the book expanded comfortably to its present
size.I hope that this extra space has allowed me to clarify
sections ofthe text that were 'heavy going' in the
earlierversion.Theextraspace has certainlyallowed me to cover
topics that were not mentioned at all in the earlier book. These
are primarily to be found in Chapters 7 and 8, which cover the
rapid advances that have been recently made in the use ofplantand
animal cells as hosts for recombinant DNAmolecules. The develop
ment ofother vectors has certainly not stood still over the past
four years.This has necessitated a thorough revision ofChapters 3
and 4, which deal with bacteriophage and bacterial plasmid vectors.
Numerous techniques for in vitromutagenesis have now been tried and
tested allowing me to givecomprehensive coverage ofthisarea in
Chapter 2 along with the biochemical techniques used to construct
recombinant DNA molecules. Readers with some background knowledge
of the approaches to gene cloning will be able to go straight
toapart ofthe book in whichthey are specificallyinterested."
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