In RNA Mapping- Methods and Protocols expert researchers in the
field detail many of the methods which are now commonly used to
study RNA. These include protocols for the consequence of the
emerging interest in the characterization of cellular RNAs urged by
their potential use as diagnostic biomarkers or therapeutic
targets. In particular, the biological relevance of microRNAs in
human physiology and disease development is highlighted in the 16
chapters focused on methods for their physical and functional
mapping. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and key
tips on troubleshooting and avoiding known pitfalls.Authoritative
and practical, RNA Mapping- Methods and Protocols provides
instruction and inspiration for scientists who are facing the
challenges of the discovery and/or functional characterization of
RNA molecules for a wide variety of applications ranging from novel
biomedical diagnostics to therapeutics and biomaterials.
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