Synthetic Messenger RNA and Cell Metabolism Modulation - Methods and Protocols (Hardcover, 2013 ed.)

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 969
Release date:	2013
First published:	2013
Editors:	Peter M. Rabinovich
Dimensions:	254 x 178 x 23mm (L x W x T)
Format:	Hardcover
Pages:	323
Edition:	2013 ed.
ISBN-13:	978-1-62703-259-9
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
LSN:	1-62703-259-2
Barcode:	9781627032599

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