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Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
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In Situ Detection of DNA Damage - Methods and Protocols (Hardcover, 2002 ed.)
Loot Price: R3,061
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In Situ Detection of DNA Damage - Methods and Protocols (Hardcover, 2002 ed.)
Series: Methods in Molecular Biology, 203
Expected to ship within 10 - 15 working days
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Detection and analysis of DNA damage is of critical importance in a
variety of biological disciplines studying apoptosis, cell cycle
and cell di- sion, carcinogenesis, tumor growth, embryogenesis and
aging, neu- degenerative and heart diseases, anticancer drug
development, environmental and radiobiological research, and
others. Individual cells within the same tissue or in cell culture
may vary in the extent of their DNA damage and, consequently, can
display different re- tions to it. These differences between
individual cells in the same cell popu- tion are detected using in
situ approaches. In situ is a Latin term meaning "on site" or "in
place." It is used to denote the processes occurring or detected in
their place of origin. In mole- lar and cell biology this usually
refers to undisrupted mounted cells or tissue sections. In that
meaning "in situ" is used as part of the terms "in situ PCR," "in
situ transcription," "in situ hybridization," "in situ end
labeling," and "in situ ligation." Sometimes the "in situ" term is
applied at the subcellular level to cells disrupted in the process
of analysis, for example, in the detection of specific sequences in
chromosomes using fluorescent in situ hybridization (FISH).
Historically, the term was used primarily in methods dealing with
nucleic acids.
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