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This volume presents techniques needed for the study of long
non-coding RNAs (lncRNAs) in cancer from their identification
to functional characterization. Chapters guide readers through
identification of lncRNA expression signatures in cancer tissue or
liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or
Nanopore Sequencing techniques; validation of lncRNA signatures by
Real time PCR, digital PCR or in situ hybridization; and functional
analysis by siRNA or CRISPR based methods for lncRNA silencing or
overexpression. Lipid based nanoparticles for delivery of siRNAs in
vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are
also treated in this volume. Written in the format of the highly
successful Methods in Molecular Biology series, each
chapter includes an introduction to the topic, lists necessary
materials and reagents, includes tips on troubleshooting and known
pitfalls, and step-by-step, readily reproducible protocols. Â
Authoritative and practical, Long Non-Coding RNAs in
Cancer aims to provide a collection of laboratory
protocols, bioinformatic pipelines, and review chapters to further
research in this vital field.Â
This volume presents techniques needed for the study of long
non-coding RNAs (lncRNAs) in cancer from their identification to
functional characterization. Chapters guide readers through
identification of lncRNA expression signatures in cancer tissue or
liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or
Nanopore Sequencing techniques; validation of lncRNA signatures by
Real time PCR, digital PCR or in situ hybridization; and functional
analysis by siRNA or CRISPR based methods for lncRNA silencing or
overexpression. Lipid based nanoparticles for delivery of siRNAs in
vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are
also treated in this volume. Written in the format of the highly
successful Methods in Molecular Biology series, each chapter
includes an introduction to the topic, lists necessary materials
and reagents, includes tips on troubleshooting and known pitfalls,
and step-by-step, readily reproducible protocols. Authoritative and
practical, Long Non-Coding RNAs in Cancer aims to provide a
collection of laboratory protocols, bioinformatic pipelines, and
review chapters to further research in this vital field.
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