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Role of miRNAs in Cancer - Analysis of Their Targetome (Hardcover): Alfons Navarro Role of miRNAs in Cancer - Analysis of Their Targetome (Hardcover)
Alfons Navarro
R1,872 R1,579 Discovery Miles 15 790 Save R293 (16%) Ships in 9 - 17 working days
Long Non-Coding RNAs in Cancer (Hardcover, 1st ed. 2021): Alfons Navarro Long Non-Coding RNAs in Cancer (Hardcover, 1st ed. 2021)
Alfons Navarro
R5,184 Discovery Miles 51 840 Ships in 10 - 15 working days

This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and practical, Long Non-Coding RNAs in Cancer aims to provide a collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field. 

Long Non-Coding RNAs in Cancer (Paperback, 1st ed. 2021): Alfons Navarro Long Non-Coding RNAs in Cancer (Paperback, 1st ed. 2021)
Alfons Navarro
R3,426 Discovery Miles 34 260 Ships in 10 - 15 working days

This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Long Non-Coding RNAs in Cancer aims to provide a collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field.

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