After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.

This collection features four peer-reviewed literature reviews on drought-resistant crops. The first chapter assesses the genes involved in drought and heat tolerance, as well as the physiological traits related to increased tolerance to abiotic stress in wheat. It reviews advances in our understanding of the molecular markers associated with these physiological traits, and the roles of key genes in determining a wheat plant's response to heat and drought stress. The second chapter discusses mechanisms of drought resistance in rice. It reviews ways of assessing drought tolerance performance, identifying genes promoting drought tolerance through quantitative trait loci (QLT) analysis, as well as breeding for drought tolerance. The third chapter highlights the genetic diversity and quantitative trait loci (QTL) associated with the traits contributing to drought and heat tolerance in soybean. It also reviews genomic resources that can facilitate a better understanding of phenotype-genotype associations and formulate genomic-assisted breeding strategies. The final chapter addresses the polygenic nature of tolerance to drought and to heat in grain legumes. It reviews advances in understanding plant adaptation to stress, the assembly of molecular and phenotyping tools to support breeding and the development of tolerance through traditional and molecular breeding techniques.

After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.