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Leading researchers and innovators describe in step-by-step detail
the latest techniques that promise to significantly impact the
practice of proteomics, as well as its success in developing novel
clinical agents. The methods span the entire spectrum of top-down
and bottom-up approaches, including microarrays, gels,
chromatography, and affinity separations, and address every aspect
of the human proteome, both quantitatively and qualitatively. The
techniques of protein detection utilized are diverse and range from
fluorescence and resonance light scattering to surface plasmon
resonance and mass spectrometry. The protocols follow the
successful Methods in Molecular Biology (TM) series format, each
offering step-by-step laboratory instructions, an introduction
outlining the principles behind the technique, lists of the
necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
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