Completely revised and expanded to reflect the most recent innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides and offers class-specific applications for the characterization and fractionation of biological macromolecules. Contains updated material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography With more than 2200 contemporary references-over 1000 more than the previous edition- HPLC of Biological Macromolecules, Second Edition is an essential source for biochemists and analytical biochemists, molecular and cell biologists, biophysicists, geneticists, chemical and biotechnological engineers, biochemical neuroendocrinologists, and upper-level undergraduate and graduate students in these disciplines.