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The present new version of this popular laboratory manual is at the
same time the first one of this text in the English language - and
this makes me even a little proud, as it reminds me of probably the
first collection of monoclonal recipes in English, written by
myself, which circulated for a couple of years in many
laboratories. In the meantime many researchers have put enormous
effort into improving methods for monoclonal antibody production.
The proce dures have become more and more standardized and by this
have more and more lost the character of magic secrets. Hinrich
Peters and Horst Baumgarten, who had followed this good tradition
already in the previous edition, written in German, suc ceeded in
making laboratory tricks teachable. They had contributed their own
experiences in cell culture and immunology, and were able to engage
a number of experienced authors to contribute to the work. They
were all willing to follow the general concept of this book, which
contains a brief theoretical background for the methods described
and presents the procedures in a highly organized structure. So the
book has retained its shape as a "cook-book," which I especially
like."
Monoklonale Antik-rper sind weit }ber die Immunologie hinaus f}r
die biomedizinische Grundlagenforschung, Diagnose und Therapie von
unsch{tzbaremWert. Ihre Herstellung stellt viele Labors zun{chst
vor gro e Probleme. Dieses Buch erm-g- licht dem Anf{nger den
Aufbau der Hybridomtechnik und gibt dem Erfahrenen vielerlei
Hinweise zur Verbesserung seines Systems. Mehrere Obersichtskapitel
vermitteln die Grundlagen der Herstellung und Charakterisierung
monoklanaler Antik-r- per. Ausf}hrliche Einzelbeschreibungen von
Methoden enthal- ten viele, bisher nicht publizierte Entwicklungen.
Damit lassen sich Klonausbeuten erheblich steigern und viele Tests
vereinfachen.
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