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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
This new volume of "Methods in Enzymology" continues the legacy
of this premier serial by containing quality chapters authored by
leaders in the field. The volumecovers ghrelin, andhas chapters on
such topics as orphan gpcrs and methods for identifying their
ligands, ghrelin o-acyltransferase assays and inhibition, and
thermogenic characterization of ghrelin receptor null mice.
By combining the tools of organic chemistry with those of physical
biochemistry and cell biology, "Non-Natural Amino" "Acids" aims to
provide fundamental insights into how proteins work within the
context of complex biological systems of biomedical interest.
Antisense technology is the ability to manipulate gene expression
within mammalian cells providing powerful experimental approaches
for the study of gene function and gene regulation. For example,
methods that inhibit gene expression permit studies which probe the
normal function of a specific product within a cell. Such
methodology can be used in many disciplines such as pharmacology,
oncology, genetics, cell biology, developmental biology, molecular
biology, biochemistry, and neurosciences. This volume will be a
truly important tool in biomedical-oriented research.
This volume supplements Volumes 63, 64, 87, and 249 of Methods in
Enzymology. These volumes provide a basic source for the
quantitative interpretation of enzyme rate data and the analysis of
enzyme catalysis. Among the major topics covered are Engergetic
Coupling in Enzymatic Reactions, Intermediates and Complexes in
Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds,
Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. More than 285 volumes have been
published (all of them still in print) and much of the material is
relevant even today-truly an essential publication for
researchersin all fields of life sciences.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. More than 260 volumes have been
published (all of them still in print) and much of the material is
relevant even today--truly an essential publication for researchers
in all fields of life sciences.
This text concerns the computer-based design and modelling, computational approaches and instrumental methods for elucidating molecular mechanisms of protein folding. Ligand-acceptor interactions are included in volumes 202 and 203 as are genetic and chemical methods for the production of functional molecules including antibodies and antigens, enzymes, receptors, nucleic acids and polysaccharides and drugs.
This volume, as does Volume 176, provides a general background of modern NMR techniques, with a specific focus on NMR techniques that pertain to proteins and enzymology, and a "snapshot" of the current state-of-the-art in NMR experimental techniques. These books enable the reader to understand a given technique, to evaluate its strengths and limitations, to decide which is the best approach, and, finally, to design an experiment using the chosen technique to solve a problem.
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