This series presents critical reviews of the present position and future trends in modern chemical research. The short and concise reports on chemistry are each written by world renowned experts. This series is still valid and useful after 5 or 10 years. More information as well as the electronic version of the whole content available at: springerlink.com.

This series presents critical reviews of the present position and future trends in modern chemical research. The short and concise reports on chemistry are each written by world renowned experts. This series is still valid and useful after 5 or 10 years. More information as well as the electronic version of the whole content available at: springerlink.com.

This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Riboregulator Design and Analysis is an ideal guide for scientists and engineers interested in the design and application of riboregulators and driving further innovation in the field.

This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Riboregulator Design and Analysis is an ideal guide for scientists and engineers interested in the design and application of riboregulators and driving further innovation in the field.

As a teen librarian, you are more likely than not to encounter teens with mental health issues. Will you know how to help them? This guide explains what to do and what not to do. Mental illness among teens has risen to epidemic levels. When mental health issues come to the library, what is the librarian's role? This book asserts that you don't have to be a social worker or mental health professional to provide guidance to teens with mental health issues. By creating collections that contain mental health resources, working with community partners, and initiating dialogues with library patrons that de-stigmatize mental illness, you can serve a positive and proactive role in helping teens to get help. This book provides readers with practical guidelines for building collections, programs, and services that support teens experiencing mental health challenges and explains how to create a supportive, welcoming environment in the library. In addition, it shows how to forge partnerships with other community agencies in this endeavor, how to advocate for mentally ill teens, and how to teach them to advocate for themselves. Lastly, it discusses how to evaluate these programs and services, and how to take care of your own needs while serving others.