This is the third volume in the "Methods in Cell Biology" series to cover protein translocation within and between cells. Volumes 31 and 32, "Vesicular Transport Parts A and B", treated techniques for eukaryotic cells available as of 1989. This text brings together new methods and topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions, transcription, crosslinking, pulse-labeling and reconstitution.

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.
Chapters reflect experimental procedures and new developments in the field of microtubule in vitro researchCombines classical approaches and modern technologiesPresents easy-to-use protocols and thorough background information, compiled by leadersin the field"

This is a cumulative index of Volumes 53-71 of the Methods in Cell Biology series. Critically acclaimed for more than 25 years, the series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.

This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques.
Key Features
* Chapters cover the commonly used video technologies in biological research
* Nontechnical presentation of principles
* Emphasis on the practical aspects of instrument use
* Covers pitfalls in instrument uses that can lead to artifacts
* Authors are leaders in the design and application of video methods to biological microscopy
* Presentation of material tailored for the established researcher that has little experience with video methods
* Examples are extensively illustrated with photographs

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.
Key Features
* Describes techniques for detection of [Ca2]]I: Ca2+ - sensitive microelectrodes
* Fluorescent dyes
* Luminescent proteins
* Includes techniques for perturbing intracellular Ca2+
* Covers detailed methodology plus problems and pitfalls of each technique
* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
* Color plates illustrate techniques such as
* Confocal ratio-imaging
* Use of aequorin

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.
Key Features
* Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes
* fluorescent dyes
* luminescent proteins
* Includes techniques for perturbing intracellular Ca2+
* Covers detailed methodology plus problems and pitfalls of each technique
* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
* Color plates illustrate techniques such as
* Confocal ratio-imaging
* Use of aequorin

Set in Germany in 1945, this is the story of a boy, Hanno, and a girl, Effi. Hanno is on the run, having just seen his twin brother killed. Effi is streetwise. She has learned the hard way that she must keep her secrets to herself - and she's even less keen to trust Hanno when she finds out he is a policeman's son. But there are far more dangerous people on the road, Russian soldiers, German deserters - and Major Otto, who likes to play games with people before he kills them. This new edition of an exceptional tale of courage, ingenuity, and the remarkable bonds formed during wartime will keep you gripped right up to the very last page.

Americana: Readings in Popular Culture, Revised Edition, is a collection of essays examining American culture from 1900 to present. Dozens of scholars investigates five aspects of our society offering unique and original insights into "What We Hear," "What We Watch," "What We Read," "Where We Go," and "The American Identity."