|
Showing 1 - 2 of
2 matches in All Departments
Many, if not all, essential biological processes require selective
interactions between proteins. Complex signaling systems require
sequential, ordered protein-protein interactions at essentially all
levels of the signaling cascade. For example, peptide hormones
interact with selective membrane receptor proteins, and
autophosphorylation of the receptor then recruits other key
regulatory proteins that initiate kinase cascades in which each
phosphorylation event requires selective recognition of the protein
substrate. The ultimate signaling effect, in many cases, is the
regulation of RNA polymerase II-directed transcr- tion in the
nucleus, a process that involves numerous, multiprotein complexes
important for transcription initiation, elongation, termination,
and reinitiation. Defining, characterizing, and understanding the
relevance of these protein- protein interactions is an arduous
task, but substantial inroads have been made over the past 20
years. The development of more recent methodologies, such as
mammalian expression systems, immunopurification schemes,
expression cloning strategies, surface plasmon resonance (BiaCore),
and nanosequencing technologies, has contributed a wealth of new
insights into these complex multiprotein mechanisms and clearly
accelerated the discovery process. Arguably, the yeast two-hybrid
system has been one of the predominant and most powerful tools in
this discovery process.
Many, if not all, essential biological processes require selective
interactions between proteins. Complex signaling systems require
sequential, ordered protein-protein interactions at essentially all
levels of the signaling cascade. For example, peptide hormones
interact with selective membrane receptor proteins, and
autophosphorylation of the receptor then recruits other key
regulatory proteins that initiate kinase cascades in which each
phosphorylation event requires selective recognition of the protein
substrate. The ultimate signaling effect, in many cases, is the
regulation of RNA polymerase II-directed transcr- tion in the
nucleus, a process that involves numerous, multiprotein complexes
important for transcription initiation, elongation, termination,
and reinitiation. Defining, characterizing, and understanding the
relevance of these protein- protein interactions is an arduous
task, but substantial inroads have been made over the past 20
years. The development of more recent methodologies, such as
mammalian expression systems, immunopurification schemes,
expression cloning strategies, surface plasmon resonance (BiaCore),
and nanosequencing technologies, has contributed a wealth of new
insights into these complex multiprotein mechanisms and clearly
accelerated the discovery process. Arguably, the yeast two-hybrid
system has been one of the predominant and most powerful tools in
this discovery process.
|
|