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Identification of differentially expressed genes is one of the
major challenges in molecular biology. Several techniques allow the
cloning of such sequences. However, methods such as RNA subtraction
or differential hybridization are time-consuming and require large
amounts of mRNA.
Recently, a new approach has successfully been developed:
Differential-Display Reverse Transcription-PCR (DDRT-PCR). This
technique has been proven to be highly effective in identifying
sequences that are differentially expressed in various cell types.
The most striking advantage is, however, that only nanograms of
total RNA are sufficient. Thus every mRNA species expressed in the
cell system can be investigated, even those present at very low
levels.
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