Differential-Display Reverse Transcription-PCR (DDRT-PCR) (Paperback, Softcover reprint of the original 1st ed. 1998)

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

General

Imprint:	Springer-Verlag
Country of origin:	Germany
Series:	Springer Lab Manuals
Release date:	2001
First published:	1998
Authors:	Sergio Colonna-Romano • Antonella Leone • Bruno Maresca
Dimensions:	235 x 155 x 7mm (L x W x T)
Format:	Paperback
Pages:	124
Edition:	Softcover reprint of the original 1st ed. 1998
ISBN-13:	978-3-540-63297-9
Categories:	Books > Science & Mathematics > Biology, life sciences > Biochemistry > General Books > Science & Mathematics > Biology, life sciences > Cellular biology > General
LSN:	3-540-63297-2
Barcode:	9783540632979

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