This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.

Few parties have undergone such comprehensive organizational change as the Labour Party since 1983. Labour's organization once institutionalized the political exchange between office-seeking politicians and the party's policy-seeking trade union paymasters. Using accessible rational choice models, Thomas Quinn explores how consecutive election defeats prompted party leaders to modernize this structure to regain voters' trust, reducing union influence in policymaking, parliamentary candidate selection and leadership contests. The price may be a centralized party vulnerable to membership exit and union funding cuts.

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Few parties have undergone such comprehensive organizational change as the Labour Party since 1983. Labour's organization once institutionalised the political exchange between office-seeking politicians and the party's policy-seeking trade union paymasters. Using accessible rational choice models, Thomas Quinn explores how consecutive election defeats prompted party leaders to modernize this structure to regain voters' trust, reducing union influence in policymaking, parliamentary candidate selection and leadership contests. The price may be a centralized party vulnerable to membership exit and union funding cuts.

Recent years have seen renewed interest in divine action, but much of the literature tends to focus on the science-theology discussion. Resulting from multi-year work of the Scripture and Doctrine Seminar, part of KLC's Scripture Collective, this book attends to the portrayal of divine action in one major biblical text, namely Hebrews. In the New Testament, Hebrews is on par with Romans in terms of importance but has too often been overlooked. Contributors to this volume explore the many different ways in which divine action is foregrounded and portrayed in Hebrews. As its name indicates, Hebrews overflows with Old Testament intertextuality, which also makes it a fertile ground for analysis of divine action stretching back into the Old Testament and opening out into different parts of the NT. The essays in this volume: rigorously work the interface of theology and exegesis, all related to Hebrews; offer an overview of the current state of discussion of divine action and the importance of exploring divine action in specific biblical texts, with special reference to William Abraham's recent 4 volume work with OUP; provide an overview of the reception history of Hebrews in theologies of divine action; explore how this has this played out in historical theology and what a retrieval of Hebrews for a theology of divine action might mean today; explore the relationship between the doctrine of God and divine action in Hebrews, including an engagement with classical theism; provocatively explore divine action in the OT, creation, and eschatology in Hebrews; explore the major theme in Hebrews of divine action through the ongoing priesthood of Jesus as portrayed in Hebrews; relate this all to preaching Hebrews today and to spiritual formation. The book's conclusion reflects on the primary action of God speaking in Hebrews.

This monograph is an account of the role of communications within the I Marine Expeditionary Force and the Marine Forces Afloat during the 1990-1991 Persian Gulf War. It is one of a series covering the operations of the I Marine Expeditionary Force; the 1st Marine Division; the 2d Marine Division; the 3d marine Aircraft Wing; Marine Combat Service Support; Marine Forces Afloat; and Marines in Operation Provide Comfort.

All aspects of pear cultivation are dealt with in this handy volume written by various experts on the subject. Thoroughly recommended reading for the budding gardener, it contains a wealth of information forming a complete how-to guide. Extensively illustrated with black and white photographs and drawings. This book contains classic material dating back to the 1900s and before. The content has been carefully selected for its interest and relevance to a modern audience.

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.