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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Neutrophil Methods and Protocols (Paperback, Softcover reprint of the original 2nd ed. 2014)
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Neutrophil Methods and Protocols (Paperback, Softcover reprint of the original 2nd ed. 2014)
Series: Methods in Molecular Biology, 1124
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Neutrophils, the most abundant white cells in humans, serve as the
primary cellular defense against infection. Neutrophil Methods and
Protocols, Second Edition provides a concise set of protocols
written by leading researchers in the field for assessing basic
neutrophil functions, investigating specialized areas in neutrophil
research, and completing step diagnostic assays of common
neutrophil disorders. Topics covered include an overview of
neutrophils and their role in host defense and inflammation;
methods most commonly used for isolating neutrophils from humans
and other animal species; procedures for subcellular fractionation
of human neutrophils, analysis of in vivo transmigrated
neutrophils, generation of mature neutrophils from induced
pluripotent stem cells and analysis of neutrophil gene expression;
methods for investigating priming, oxidant production,
phagocytosis, bactericidal activity and extracellular trap
formation and protocols for investigating neutrophil adhesion,
chemotaxis and outside-in signaling via integrins. Written in the
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible, Neutrophil
Methods and Protocols, Second Edition is a comprehensive source for
detailed explanations and applications of the most modern
methodological advances in neutrophil biology. Both basic
scientists and clinicians will find a collection of this caliber to
be an invaluable aid in their work with neutrophils.
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