For those who could read between the lines, the censored news out of China was terrifying. But the president insisted there was nothing to worry about. Fortunately, we are still a nation of skeptics. Fortunately, there are those among us who study pandemics and are willing to look unflinchingly at worst-case scenarios.

Michael Lewis’s taut and brilliant nonfiction thriller pits a band of medical visionaries against the wall of ignorance that was the official response of the Trump administration to the outbreak of COVID-19. The characters you will meet in these pages are as fascinating as they are unexpected.

A thirteen-year-old girl’s science project on transmission of an airborne pathogen develops into a very grown-up model of disease control. A local public-health officer uses her worm’s-eye view to see what the CDC misses, and reveals great truths about American society. A secret team of dissenting doctors, nicknamed the Wolverines, has everything necessary to fight the pandemic: brilliant backgrounds, world-class labs, prior experience with the pandemic scares of bird flu and swine flu…everything, that is, except official permission to implement their work.

Michael Lewis is not shy about calling these people heroes for their refusal to follow directives that they know to be based on misinformation and bad science. Even the internet, as crucial as it is to their exchange of ideas, poses a risk to them. They never know for sure who else might be listening in.

Collins Big Cat supports every primary child on their reading journey from phonics to fluency. Top authors and illustrators have created fiction and non-fiction books that children love to read. Levelled for guided and independent reading, each book includes ideas to support reading. Teaching and assessment support and eBooks are also available. When Tara Binns opens her dressing up box something exciting happens ... Tara Binns is a microbiologist! Something is causing chaos in the scientists' greenhouse ... and everything Tara touches seems to fall apart! Can she corner the culprit and get to the root of the problem? This exciting title in the Tara Binns mini-series is written by Lisa Rajan. Copper/Band 12 books provide more complex plots and longer chapters that develop reading stamina. Text type: An adventure story Ideas for reading in the back of the book provide practical support and stimulating activities.

Please note this version is for instructors only; students and bookstores should purchase 9780815346102. As with the first edition, this new edition of Living in a Microbial World is written for students taking a general microbiology course, or a microbiology-based course for non-science majors. The conversational style and use of practical, everyday examples make the essential concepts of microbiology accessible to a wide audience. While using this approach, the text maintains scientific rigor with clear explanations spanning the breadth of microbiology, including health, evolution, ecology, food production, biotechnology, and industrial processes. Each chapter contains a series of case studies based on microbiology in the news, in history, and in literature. There are questions at the end of each case study and the end of each chapter, as well as an online quiz with help on answering the questions. The text, questions, and cases have been updated to reflect the changing influence of microbiology in the world today, from the microbiome, to new disease outbreaks (Ebola and Zika) and antibiotic resistance, to new biotechnology tools (CRISPR-Cas). Living in a Microbial World, Second Edition is additionally supported by the Garland Science Learning System. This homework platform is designed to evaluate and improve student performance and allows instructors to select assignments on specific topics and review the performance of the entire class, as well as individual students, via the instructor dashboard. Students receive immediate feedback on their mastery of the topics, and will be better prepared for lectures and classroom discussions. The user-friendly system provides a convenient way to engage students while assessing progress. Performance data can be used to tailor classroom discussion, activities, and lectures to address students' needs precisely and efficiently. For more information and sample material, visit http://garlandscience.rocketmix.com/. Living in a Microbial World, Second Edition comes with a full range of supplements: The Garland Science Learning System Images available in PowerPoint and JPEG Testbank Online quiz with answers and feedback Extra modules Help answering the end-of-chapter questions Animations Media guide Online glossary

Microbial quality of water is a prime public health concern in today's world. To protect public health, the World Health Organization and the U.S. Environmental Protection Agency have established microbial pollution indicator standards and recommended routine monitoring of water for both total and fecal coliforms (Dufour, 1984, U.S. EPA 1986,1994, WHO 1993). However, the adequacy of current water quality standards to indicate the presence or absence of human pathogens is still questionable. For example, human viruses are more resistant to sewage treatment processes and environmental conditions than bacterial indicators and therefore may pose a substantial threat. It is now recognized that the absence, or a low concentration, of indicator organisms in water may not adequately reflect the absence of human viruses. In our previous study of southern California coastal waters, we found over 30% of coastal waters tested contained human viruses, and the presence of these viruses did not correlate with an elevated level of bacterial indicators (Jiang et al. 2000). The goal of this research is to develop and validate a molecular method for rapid and specific detection of microbial contaminants including human viruses and bacterial indicators in treated sewage effluents and receiving waters. Research Outcomes An extensive search and review of current state of technology for molecular alternatives to indicator and viral pathogen detection was conducted. The results indicate that although real-time PCR methods have been widely applied in the clinical research for detection of human viruses, environmental application of this method is very limited. In addition, there is an urgent need for a method for efficient concentration and purification of human viruses in complex environmental matrixes. During this study, we have designed, tested and optimized real-time quantitative PCR method using specific and degenerate primers and probes targeting at adenoviruses and enterococci, respectively. Experimental testing of real time PCR primers and probes for adenoviruses demonstrated reproducible results at efficiency greater than 90% over a 5-log dynamic range of target concentration. The enterococci real time PCR was efficient at 99% of the time and over a 7-log dynamic range of target concentration. Application of these methods to sewage effluents and coastal waters demonstrated that real-time PCR methods are more sensitive than culturing methods at detection of targets, suggesting a great potential for real-time quantification of microbial contaminants in environments. However, real-time PCR based method, like other genome based detection technology, overestimates the concentration of infectious viral concentration in the environment. In addition to the method development for real-time PCR detection, we have also sampled and isolated human adenoviruses from Newport Bay, California using human embryonic kidney cells, 293A. Cloning and sequencing of selective environmental adenovirus hexon gene suggested that most of the viruses recovered from the environment belong to adenovirus serotype 40 (66%). This result is in agreement with clinical data on the load of viral shedding in feces. Since adenovirus serotypes 40 and 41 are the major cause of childhood diarrhea, the result of this investigation indicates the importance of monitoring water for viral quality. Comparison of four different tissue culture cell lines for their sensitivities to adenoviruses infection has demonstrated that the genetically engineered 293A cells are the most efficient at recovery of adenoviruses 40. This result is a significant contribution to our ability to assay for infectious adenoviruses in environmental samples.

Basic and applied microbiology gives a fresh perspective on microbiology. It deals with some of the important issues of the day, including genetically modified food; the increased incidence of food- and waterborne diseases and their control; the introduction of HACCP legislation worldwide; microbial resistance to antimicrobial compounds and the development of multiple drug-resistant organisms; the alleviation of environmental pollution using bioremediation and biofouling; and biocorrosion in water systems, to mention just a few. The title is supported by an e-learning platform with a comprehensive set of animations explaining the basic concepts. The Web portal accompanying the book also provides a gateway to carefully selected Internet sites, unlocking the world of microbiology for the experienced microbiologist and the uninitiated alike.

Drinking water provides an efficient source for the spread of gastrointestinal microbial pathogens capable of causing serious human disease. The massive death toll and burden of disease worldwide caused by unsafe drinking water is a compelling reason to value the privilege of having safe drinking water delivered to individual homes. On rare occasions, that privilege has been undermined in affluent nations by waterborne disease outbreaks traced to the water supply. Using the rich and detailed perspectives offered by the evidence and reports from the Canadian public inquiries into the Walkerton (2000) and North Battleford (2001) outbreaks to develop templates for understanding their key dimensions, over 60 waterborne outbreaks from 15 affluent countries over the past 30 years are explored as individual case studies. Recurring themes and patterns are revealed and the critical human dimensions are highlighted suggesting insights for more effective and more individualized preventive strategies, personnel training, management, and regulatory control. Safe Drinking Water aims to raise understanding and awareness of those factors that have most commonly contributed to or caused drinking-water-transmitted disease outbreaks - essentially a case-history analysis within the multi-barrier framework. It contains detailed analysis of the failures underlying drinking-water-transmitted disease epidemics that have been documented in the open literature, by public inquiry, in investigation reports, in surveillance databases and other reliable information sources. The book adopts a theme of 'converting hindsight into foresight', to inform drinking-water and health professionals including operators, managers, engineers, chemists and microbiologists, regulators, as well as undergraduates and graduates at specialty level. Key Features: Contains details and perspectives of major outbreaks not widely known or understood beyond those directly involved in the investigations. Technical and scientific background associated with case studies is offered in an accessible summary form. Does not require specialist training or experience to comprehend the details of the numerous outbreaks reviewed. By providing a broad-spectrum review using a consistent approach, several key recurring themes are revealed that offer insights for developing localized, tailor-made prevention strategies.

Since the identification of the first cases of the coronavirus in December 2019, there has been a significant amount of confusion regarding the origin and spread of the so-called 'coronavirus', SARS-CoV-2, and the cause of the disease COVID-19. Conflicting messages from the media and officials across different countries and organizations, the abundance of disparate sources of information, unfounded conspiracy theories on the origins of the virus, unproven therapies, and inconsistent public health measures, have all served to increase anxiety in the population. Where did the virus come from? How is it transmitted? How does it cause disease? Is it like flu? What is a pandemic? In this concise and accessible introduction, a leading expert provides answers to these commonly asked questions. This revised and updated edition now also covers how the virus mutates, how important these mutations are, how vaccines work, and what we can expect in the near and long-term future.

This updated text collects all the introductory aspects of beer brewing science into one place for undergraduate brewing science courses. This expansive and detailed work is written in conversational style, walking students through all the brewing basics from the origin and history of beer to the brewing process to post-brew packaging and quality control and assurance. As an introductory text, this book assumes the reader has no prior knowledge of brewing science and only limited experience with chemistry, biology and physics. The text provides students with all the necessary details of brewing science using a multidisciplinary approach, with a thorough and well-defined program of in-chapter and end-of-chapter problems. As students solve these problems, they will learn how scientists think about beer and brewing and develop a critical thinking approach to addressing concerns in brewing science. As a truly comprehensive introduction to brewing science, Brewing Science: A Multidisciplinary Approach, Second Edition walks students through the entire spectrum of the brewing process. The different styles of beer, the molecular makeup and physical parameters, and how those are modified to provide different flavors are listed. All aspects of the brewery process, from the different setup styles to sterility to the presentation of the final product, are outlined in full. All the important brewing steps and techniques are covered in meticulous detail, including malting, mashing, boiling, fermenting and conditioning. Bringing the brewing process full circle, this text covers packaging aspects for the final product as well, focusing on everything from packaging technology to quality control. Students are also pointed to the future, with coverage of emerging flavor profiles, styles and brewing methods. Each chapter in this textbook includes a sample of related laboratory exercises designed to develop a student's capability to critically think about brewing science. These exercises assume that the student has limited or no previous experience in the laboratory. The tasks outlined explore key topics in each chapter based on typical analyses that may be performed in the brewery. Such exposure to the laboratory portion of a course of study will significantly aid those students interested in a career in brewing science.

The book elucidates the role of inorganic polyphosphates in eukaryotic cells, from fungi and protozoa to human being. To date, there is plenty of evidence that these anionic biopolymers occurring in the cells of all living organisms, from bacteria to humans, perform numerous regulatory functions. The book describes the evolution of PolyPs, their role in lower eukaryotes and their involvement in various processes in the human organism, as well as its use in biomaterials such as bioactive glass and engineered bone tissue. The aim of this book is to summarize the data of the past decade on the functional role of inorganic polyphosphates in eukaryotes and discuss their biological role also in context of common human diseases. The book will provide a modern concept of the functional significance of these biopolymers, useful for researchers in cell biology, biochemistry, molecular biology and biomedicine alike.

This book is a compilation of detailed articles on various products and services that can be derived from bioresources through bioprocess. It offers in-depth discussions and case studies on commercially and therapeutically important enzymes, antimicrobials, anti-cancer molecules and anti-inflammatory substances. It also includes a separate section on emerging trends in bioactive substances research. This unique book is a valuable source of information for biotechnologists and bioprocess experts as well as academics and researchers who are actively involved in product and process development.

Since the identification of the first cases of the coronavirus in December 2019, there has been a significant amount of confusion regarding the origin and spread of the so-called 'coronavirus', SARS-CoV-2, and the cause of the disease COVID-19. Conflicting messages from the media and officials across different countries and organizations, the abundance of disparate sources of information, unfounded conspiracy theories on the origins of the virus, unproven therapies, and inconsistent public health measures, have all served to increase anxiety in the population. Where did the virus come from? How is it transmitted? How does it cause disease? Is it like flu? What is a pandemic? In this concise and accessible introduction, a leading expert provides answers to these commonly asked questions. This revised and updated edition now also covers how the virus mutates, how important these mutations are, how vaccines work, and what we can expect in the near and long-term future.

This book is a compilation of case studies from different countries and covers contemporary with future prospective for sustainable development of agriculture. The book highlights the real-world as well as future generation situations facing the challenges for the twenty first century will be production of sufficient food and highlights the strengths, weaknesses and opportunities, to meet the needs of fast growing population it is imperative to increase agricultural productivity in an environmentally sustainable manner. Due to imbalanced use of chemical fertilizers and agrochemicals has a considerable negative impact on economy and environmental sustainability of nation, for the sustainable alternative means to solve these problems, the efficient utilization of biological agents have been extensively studied. Naturally existing plant-microbe-environment interactions are utilized in many ways for enhancing plant productivity. A greater understanding of how plants and microbes live together and benefit each other can therefore provide new strategies to improve plant productivity, in most sustainable way. To achieve the objective of sustainable agricultural practices there is a need for understanding both basic and applied aspects of agriculturally important microorganisms. Focus needs to be on transforming agricultural systems from nutrient deficient to nutrient rich soil-plant system. This book is split into two parts, with an aim to provide comprehensive description and highlight a holistic approach. It elucidated various mechanisms of nutrients solubilisation and its importance in enhancement of plant growth, nutrient content, yield of various crops and vegetables as well as soil fertility and health. Unit-1 in this book explains the importance of soil microbes in sustainable crop production. It contains chapters detailing the role and mechanism of action of soil microbes which enhances the productivity via various bio-chemical and molecular channe ls. In unit-2 the role of microbes in plant protection is elaborated. With the help of case studies of food crops, multiple ways in which soil microbes help in fighting and preventing plant diseases is explained. With the given content and layout book will be an all-inclusive collection of information, which will be useful for students, academicians, researchers working in the field of rhizospheric mechanisms, agricultural microbiology, soil microbiology, biotechnology, agronomy and sustainable agriculture and also for policy makers in the area of food security and sustainable agriculture.

This book reviews the potential mechanisms in arbuscular mycorrhizas (AMs), in the hope that this can help arbuscular mycorrhizal fungi (AMF) to be more used efficiently as a biostimulant to enhance stress tolerance in the host plants. AMF, as well as plants, are often exposed to all or many of the abiotic and biotic stresses, including extreme temperatures, pH, drought, water-logging, toxic metals and soil pathogens. Studies have indicated a quick response to these stresses involving several mechanisms, such as root morphological modification, reactive oxygen species change, osmotic adjustment, direct absorption of water by extraradical hyphae, up-regulated expression of relevant stressed genes, glomalin-related soil protein release, etc. The underlying complex, multi-dimensional strategy is involved in morphological, physiological, biochemical, and molecular processes. The AMF responses are often associated with homeostatic regulation of the internal and external environment, and are therefore critical for plant health, survival and restoration in native ecosystems and good soil structure.

Geomicrobiology is the study of microbes and microbial processes and their role in driving environmental and geological processes at scales ranging from the nano, micron, to meter scale. This growing field has seen major advances in recent years, largely due to the development of new analytical tools and improvements to existing techniques, which allow us to better understand the complex interactions between microbes and their surroundings. In this comprehensive handbook, expert authors outline the state-of-the-art and emerging analytical techniques used in geomicrobiology. Readers are guided through each technique including background theory, sample preparation, standard methodology, data collection and analysis, best practices and common pitfalls, and examples of how and where the technique has been applied. The book provides a practical go-to reference for advanced students, researchers and professional scientists looking to employ techniques commonly used in geomicrobiology.

This book is a compilation of articles on various aspects of bioresources and the processes employed for its judicious utilization. Biodiversity and conservation, food security, gene banks and repositories, laws governing biodiversity, bioprospecting, bioresources in traditional medicine and biodiversity mining are some of the important topics covered in the book. The unique contents of the book make it an important source of information for conservation scientists, academics, activists and to those who are actively involved in product oriented research from bioresources.

The book addresses current public concern about the adverse effect of agrochemicals and their effect on the agro-ecosystem. This book also aims to satisfy and contribute to the increasing interest in understanding the co-operative activities among microbial populations and their interaction with plants. It contains chapters on a variety of interrelated aspects of plant-microbe interactions with a single theme of stress management and sustainable agriculture. The book will be very useful for students, academicians, researcher working on plant-microbe interaction and also for policy makers involved in food security and sustainable agriculture.

Many creatures use adhesive polymers and structures to attach to inert substrates, to each other, or to other organisms. This is the first major review that brings together research on many of the well-known biological adhesives dealing with bacteria, fungi, algae, and marine and terrestrial animals. As we learn more about their molecular and mechanical properties we begin to understand why they adhere so well and with this comes broad applications in areas such as medicine, dentistry, and biotechnology.

This book recounts the biology of M. bovis, followed by the status of bovine Tuberculosis (bTB) in African countries, primarily based on zoonotic and epidemiological field reports. Since the accumulation of data is valueless unless it led to practicable control measures, emphasis is put on locally adapted protocols for future control of the disease. In order to systematically evaluate the knowledge base of bTB, Epidemiologic Problem Oriented Approach (EPOA) methodology was used. The methodology is composed of two triads: i) the problem identification/characterization triad, which is mainly descriptive in nature, and ii) the problem management/solution/mitigation triad, which is mainly geared toward problem management/solution (see figure). The first triad comprises three pillars: i) agent ii) host, and iii) environment and the second one: i) therapeutics/treatment, ii) prevention/control, and iii) health maintenance/promotion. The two triads are linked together by the diagnostic procedure linkage. The systematic and detailed studies of the 'Host-Agent-Environment' interactions are the building blocks to the understanding of agent transmission pathways and disease spread. These may include data about the disease status of the country, the nature of the disease agent and its hosts, the modes of transmission, the wildlife reservoirs in nature, persistence of infection, and agent survival in animal products and the environment. The problem identification and characterization triad identifies these interactions. Once a problem has been identified and well understood, the next step is to minimize the risk of transmission and spread of a disease. This area, referred to as problem solution/management triad, consists of problem management alternatives that rely upon prevention/control, and health maintenance/promotion of the disease in livestock, wildlife, and humans with the emphasis on resource-poor, developing countries in Africa.

This book presents a state-of-the-art compilation of articles on the role of non-coding RNAs as pivotal molecules for establishing functional relationships between different organisms. It also describes how non-coding RNA molecules can act as dynamic communication devices and how they modulate the interaction between different kingdoms. It describes examples including viruses, bacteria, fungi, and higher organisms such as protozoa that are involved in pathogenic or symbiotic interactions, and the roles of different families of non-coding RNAs within these relationships. Each chapter provides readers molecular and functional insights, breaking down the subjacent mechanisms and exploring their functional implications and future applications. More than sixty years after the foundation of the "RNA tie club" by George Gamow, new roles for non-coding RNA molecules are starting to be revealed.

This book examines how the growing knowledge of the huge range of animal-bacterial interactions, whether in shared ecosystems or intimate symbioses, is fundamentally altering our understanding of animal biology. Individuals from simple invertebrates to human are not solitary, homogenous entities but consist of complex communities of many species that likely evolved during a billion years of coexistence. Defining the individual microbe-host conversations in these consortia, is a challenging but necessary step on the path to understanding the function of the associations as a whole. The hologenome theory of evolution considers the holobiont with its hologenome as a unit of selection in evolution. This new view may have profound impact on understanding a strictly microbe/symbiont-dependent life style and its evolutionary consequences. It may also affect the way how we approach complex environmental diseases from corals (coral bleaching) to human (inflammatory bowel disease etc). The book is written for scientists as well as medically interested persons in the field of immunobiology, microbiology, evolutionary biology, evolutionary medicine and corals.

This book highlights the efforts made by distinguished scientific researchers world-wide to meet two key challenges: i) the limited reserves of polluting fossil fuels, and ii) the ever-increasing amounts of waste being generated. These case studies have brought to the foreground certain innovative biological solutions to real-life problems we now face on a global scale: environmental pollution and its role in deteriorating human health. The book also highlights major advances in microbial metabolisms, which can be used to produce bioenergy, biopolymers, bioactive molecules, enzymes, etc. Around the world, countries like China, Germany, France, Sweden and the US are now implementing major national programs for the production of biofuels. The book provides information on how to meet the chief technical challenges - identifying an industrially robust microbe and cheap raw material as feed. Of the various possibilities for generating bioenergy, the most attractive is the microbial production of biohydrogen, which has recently gained significant recognition worldwide, due to its high efficiency and eco-friendly nature. Further, the book highlights factors that can make these bioprocesses more economical, especially the cost of the feed. The anaerobic digestion (AD) process is more advantageous in comparison to aerobic processes for stabilizing biowastes and producing biofuels (hydrogen, biodiesel, 1,3-propanediol, methane, electricity), biopolymers (polyhydroxyalkanoates, cellulose, exopolysaccharides) and bioactive molecules (such as enzymes, volatile fatty acids, sugars, toxins, etc.) for biotechnological and medical applications. Information is provided on how the advent of molecular biological techniques can provide greater insights into novel microbial lineages. Bioinformatic tools and metagenomic techniques have extended the limits to which these biological processes can be exploited to improve human welfare. A new dimension to these scientific works has been added by the emergence of synthetic biology. The Big Question is: How can these Microbial Factories be improved through metabolic engineering and what cost targets need to be met?

This book is a comprehensive field guide to the mushrooms of the southeastern United States. Although it will stand on its own, it is intended to compliment and serve as a companion to Mushrooms of Northeastern North America, also published by Syracuse University Press. Together these volumes form a foundation and reference for identifying mushrooms found in eastern North America from Canada to the subtropics of Florida and Texas. This book features more than 450 species that are fully described and illustrated with photographs, many for the first time in color. The photographs were selected for high-quality color fidelity and documentary merit, and reflect some of the aesthetic appeal of our subject. The number of species described and illustrated in color is substantially more than has previously appeared in any other single work devoted to the mushrooms of the southeastern United States. Cross referencing to additional species occuring in the region that are illustrated in Mushrooms of Northeastern North America is provided. Although this book contains the necessary detail required by advanced students and professional mycologists, it emphasizes identification based primarily on macroscopic field characters for easier use by a general audience. Each illustrated species is accompanied by a detailed description of macroscopic and microscopic features based on the concepts of their original authors.

The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea

This book deals with the noteworthy advancement in the production of bioactive metabolites from microbes and their pharmacological significance. It highlights the pharmacological potential of marine microbes and endophytic fungi and their bioactive secondary metabolites. Emphasis is also given on the significance of probiotics and their specialized molecules in human health and disease as well as their role in dietary intervention for reducing the risk of non-alcoholic fatty liver disease. This work also serves as excellent reference material for researchers, students and academicians in the field of natural product chemistry, pharmacology and applied microbiology.