Somatic genome manipulation is required when a sexual crossing approach cannot be used in breeding or genetic treatment of an individual organism. Examples can include gene- or cell-therapy of a person to correct disease, genetic improvement of vegetatively propagated plants, and genetic replacement of cytoplasm without significantly modifying the nuclear genome. The advantage of somatic genome manipulation is maintenance of the general genotype while correcting one or more traits. Somatic genome manipulation is also an option for genetic improvement of sexually propagated plants in polyploidy breeding or in overcoming issues of sexual incompatibility. Recent novel technologies in somatic genome manipulation are developing quickly but much of this literature is fragmented and difficult or inconvenient to access. This book represents the first attempt to assemble updated reviews, detailed protocols, and their applications in all fields in which somatic genome manipulation has thrived. This is a truely one-of-a-kind work that brings together the most important and relevant advances in somatic genome manipulation in plants, algae, microorganisms, humans and animals, and demonstrates where the science interacts and where it diverges. The chapters are written by experts on the topic with ready-to-use protocols that were originally developed or adapted from the literature in their laboratories. We expect that this book will be useful for students, researchers, and teachers in both plant and animal research as a resource for the latest information on somatic genome manipulation and for its useful laboratory methods.

Microorganisms are ubiquitous and indispensable for the existence of mankind. They show diversity in size, shape, metabolism and the range of positive functions they perform for sustaining the life on this planet. Bacteria have been exploited by the mankind since times immemorial for the production of various foods and enzymes. They reveal several types of metabolic reactions which are absent in eukaryotic organisms. The present book highlights the potential of microorganisms in solving the global energy crisis. Presently, the world is facing energy crisis due to depleting fossil fuels which are expected to get exhausted during the next 50 yeaOne of the alternative energy resources for the new millennium is expected to be the renewable energy including biomass from which a variety of biofuels can be obtained by the exploitation of microbes. This volume has been organized in 13 s which have been prepared to provide the readers with both an in-depth study and a broad perspective of microorganisms for sustainability of mankind. Further, it makes the readers familiar with the diversity in energy generating pathways among different groups of microorganisms and different types of biomass energy resources available on this planet and the various possibilities which can be exploited for converting these in to alternate energy sources with the help of microbes. A great effort has been made to provide the readers a comprehensive knowledge about different alternative fuels and value added products from microbes for the 21st century. It is hoped that this volume will prove useful to the students and professionals who are pursuing their career in Microbiology, Biotechnology, Biochemistry, Environmental sciences and Energy studies related to the alternate biofuels to solve the global energy crisis.

The book is written for the college students to provide wide information about the fundamental aspects of microbiology. The book is designed in such a manner to understand all the basics, principles and recent trends in the field of microbiology. Enough diagrams and pictures are given then and there to understand the chapteIt also covers new concepts in microbiology such as environmental microbiology and biotechnology etc.

The rapid increase in microbial resources along with the development of biotechnological methods has revolutionized the field of microbial biotechnology. Genome characterization methods and metagenomic approaches further illustrate the role of microorganisms in various fields of research. Recent Advancement in Microbial Biotechnology: Agricultural and Industrial Approach provides an overview on the recent application of the microorganisms in agricultural and industrial improvements. The purpose of this book is to integrate all these diverse areas of research in a common platform. Recent advancement in Microbial Biotechnology targets researchers from both academia and industry, professors and graduate students working in molecular biology, microbiology and biotechnology.

In the past two decades, several pandemics have ravaged the globe, giving us several lessons on infectious disease epidemiology, the importance of initial detection and characterization of outbreak viruses, the importance of viral epidemic prevention steps, and the importance of modern vaccines. Pandemic Outbreaks in the Twenty-First Century: Epidemiology, Pathogenesis, Prevention, and Treatment summarizes the improvements in the 21st century to overcome / prevent / treat global pandemic with future prospective. Divided into 9 chapters, the book begins with an in-depth introduction to the lessons learned from the first pandemic of the 21st century. It describes the history, present and future in terms of detection, prevention and treatment. Followed by chapters on the outbreak, treatment strategies and clinical management of several infectious diseases like MERS, SARD and COVID 19, Pandemic Outbreaks in the Twenty-First Century: Epidemiology, Pathogenesis, Prevention, and Treatment, presents chapters on immunotherapies and vaccine technologies to combat pandemic outbreak and challenges. The book finishes with a chapter on the current knowledge and technology to control pandemic outbreaks. All are presented in a practical short format, making this volume a valuable resource for very broad academic audience.

This book covers intentional design aspects for combinations of drugs, single-molecule hybrids with potential or actual multiple actions, pro-drugs which could yield multiple activity outcomes, and future possibilities. The approach of the book is interdisciplinary, and it provides greater understanding of the complex interplay of factors involved in the medicinal chemistry design and laboratory development of multiply active antibacterials. The scope of the book appeals to readers who are researching in the field of antibacterials using the approach of medicinal chemistry design and drug development.

Published since 1959, "Advances in Applied Microbiology" continues to be one of the most widely read and authoritative review sources in microbiology. The series contains comprehensive reviews of the most current research in applied microbiology. Recent areas covered include bacterial diversity in the human gut, protozoan grazing of freshwater biofilms, metals in yeast fermentation processes and the interpretation of host-pathogen dialogue through microarrays. Eclectic volumes are supplemented by thematic volumes on various topics, including Archaea and sick building syndrome. Impact factor for 2011: 5.233.

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Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

Natural phenolics are powerful bioactive compounds, but their use as antioxidant agents in lipid-based foodstuffs and cosmetics is limited due to their hydrophilic traits. A promising technique to overcome low solubility of phenolics is to increase their hydrophobicity by grafting with lipophilic moiety to form lipid-enriched phenolics (lipo-phenolics). Another way to enhance the amphiphilic traits of phenolics is by lipophilization with phospholipids in a suitable solvent to form phenolics-enriched phospholipids (pheno-phospholipids). Both functionalized phenolics (phenolipids) exhibit high bioavailability and antioxidative potential. Functional phenolics-enriched phospholipids (pheno-phospholipids) play an important role in enhancing the functional properties of both phenolic compounds and phospholipids in food for their use in nutrition and health. Phenolipids have also found applications on an industrial scale, likely due to low costs, the availability of starting material and safety. Recent advances in the field of lipophilization allow accessing molecules with high potency and targeted action covering a wide spectrum of bioactivities. Owing to their cost and availability, phenolipids find applications in niche sectors such as cosmetics and pharmaceutics as well as in the novel food. This book reports on the chemistry, preparation, and functionality of lipid-enriched phenolics (lipo-phenolics), broadening their applications in food, pharmaceuticals and cosmetics. The strategies of the lipophilization of phenolics, the effect of modification on the biological properties and potential applications of the resulting lipo-phenolics are reviewed. The text also discusses the preparation, physicochemical characteristics and functional properties of phenolipids and phytosomes, including the latest developments and their current industrial status.

This book examines the commercial role of various microbial polysaccharides and recent advances in their production. Offering an overview of the physiological role, biosynthetic pathways and regulatory mechanisms, it also explores the current challenges regarding bioprocessing for the production of polysaccharides.

Biofilms are the default mode-of-life for many bacterial species. The three-dimensional structure of the biofilm provides the associated microbial communities with additional protection from predation, toxic substances, and physical perturbation. The variety of microniches provided by the biofilm also promotes a huge diversity of microbial life and metabolic potential. These complex and highly structured communities help to maintain the health of soils and waters. Current applications of biofilms include the degradation of toxic substances in soil and water, the commercial production of chemicals, and the generation of electricity. However, biofilm-based infections cause harm to millions of humans annually. In addition, biofilms can affect the quality and yield of crops and cause biofouling and microbially-induced corrosion. In this book, leading scientists provide an up-to-date review of the latest scientific research on these fascinating microbial communities and predict future trends and growth areas in biofilm-related research. The book will be essential for everyone interested in biofilms and their applications. It is also highly recommended for environmental microbiologists, soil scientists, medical microbiologists, bioremediation experts, and microbiologists working in biocorrosion, biofouling, biodegradation, water microbiology, quorum sensing, and many other areas.

Written by a team of acknowledged experts, this book provides an up-to-date overview of our current understanding of bacterial glycomes, describes the main analytical methods in use and discusses recent and novel applications. The book is divided into three sections. The first section includes overviews of microbial glyconjugates, lipopolysaccharide, capsular polysaccharide, lipoarabinomannan biosynthesis, cell wall metabolism, and glycosylation of bacterial and archaeal proteins. The second section reviews the analytical approaches used in the characterization of the bacterial glycome. The final section describes applications of bacterial glycomics, including metabolic oligosaccharide labeling, the synthesis of bioactive glycans and the potential for glycoconjugate vaccines.

This book complies latest advancement in the field of environmental biotechnology. It focuses on topics that comprises industrial, environment and agricultural related issues to microbiological studies and exhibits correlation between biological world and dependence of humans on it. It is designed into three sections covering the role of environmental biotechnology in industry, environmental remediation, and agriculture. Ranging from micro-scale studies to macro, it covers up a huge domain of environmental biotechnology. Overall the book portrays the importance of modern biotechnology technologies in solving the problems in modern day life. The book is a ready reference for practicing students, researchers of biotechnology, environmental engineering, chemical engineering and other allied fields likewise.

This book emphasizes and presents the latest information on eco-physiology and biochemistry of cyanobacteria with special emphasis on their biodiversity, molecular mechanisms of some important biological processes and survival mechanisms under myriad of environmental conditions as well as bioremediation. Cyanobacteria are the most dominant prokaryotic floras on the Earth's surface, and are of great importance in terms of ecological, economical and evolutionary perspectives. They are oldest groups of photosynthetic autotrophs, which create oxygenic atmosphere for the development and sustainability of ecosystems with different life forms. The book presents an integrative approach to their possible biotechnological application in the field of bio-energy and various aspects of biochemistry, biophysics and structural biology of photosynthesis. The various chapters describe the different applications of cyanobacteria as bio-energy sources and in phycoremediation. The contents incorporated in this book can be used as a textbook by undergraduate and post-graduate students, teachers, and researchers in the most interesting fields of physicochemical ecology and biochemistry of cyanobacteria.

For courses in Microbiology Lab and Nursing and Allied Health Microbiology Lab A Flexible Approach to the Modern Microbiology Lab Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the Eleventh Edition incorporates current safety protocols from governing bodies such as the EPA, ASM, and AOAC. The new edition also includes alternate organisms for experiments for easy customisation in Biosafety Level 1 and 2 labs. New lab exercises have been added on Food Safety and revised experiments, and include options for alternate media, making the experiments affordable and accessible to all lab programs. Ample introductory material, engaging clinical applications, and laboratory safety instructions are provided for each experiment along with easy-to-follow procedures and flexible lab reports with review and critical thinking questions.

The rapid urbanization and industrialization of developing countries across the globe have necessitated for substantial resource utilization and development in the areas of Healthcare, Environment, and Renewable energy. In this context ,this resourceful book serves as a definitive source of information for the recent developments in application of microbial enzymes in various sectors. It covers applications in fermentation processes and their products, extraction and utilisation of enzymes from various sources and their application in health and biomass conversion for production of value added products. Different chapters discuss various areas of bioprospecting in enzyme technology, and describe why these are the mainstays for industrial production of value added products. The rich compilation of the cutting-edge advances and applications of the modern industrial based techniques hold feasible solutions for a range of current issues in enzyme technology. This book will be of particular interest for scientists, academicians, technical resource persons, engineers and members of industry. Undergraduate and graduate students pursuing courses in the area of industrial biotechnology will find the information in the book valuable. General readers having interest towards biofuels, enzyme technology, fermented food and value added products, phytochemicals and phytopharmaceutical products will also find the book appealing. Readers will discover modern concepts of enzymatic bioprocess technology for production of therapeutics and industrial value added products.

This book provides a comprehensive account of past, present and future of the biomass based biorefineries. It is an all-inclusive and insightful compilation of recent advancements in the technology and methods used for conversion of biomass to bioenergy and other useful biochemicals. The book also focuses on the limitations of existing technologies and provides the future prospects, as well as discusses socio-economic impact of biomass based biorefineries. This book assists researchers in the area of lignocellulosic biorefineries and can be used by the students, scientist and academician as an advanced reference textbook.

This book provides up-to-date information on the state of the art in applications of biotechnological and microbiological tools for protecting the environment. Written by leading international experts, it discusses potential applications of biotechnological and microbiological techniques in solid waste management, wastewater treatment, agriculture, energy and environmental health. This second volume of book "Environmental Microbiology and Biotechnology," covers two main topics: bioenergy and environmental health, exploring the latest developments from around the globe regarding applications of biotechnology and microbiology for converting wastes into valuable products and at the same time reducing the environmental pollution resulting from disposal. Wherever possible it also includes real-world examples. Further, it offers advice on which procedures should be followed to achieve satisfactory results, and provides insights that will promote the transition to the sustainable utilization of various waste products.

This book compiles the latest research on the multifarious roles of microbial enzymes, and provides an overview of microbial enzymes and biotechnologies. It discusses the use of microbial enzymes in innovative areas like nanomedicine and synthetic biotechnology, as well as the use of starch digesting enzymes and bioactive proteins as biotherapeutics, all of which have applications in modern drug discovery processes. The book also examines the concept of microbial biotransformation and protein engineering, and covers topics such as the immobilization of therapeutic enzymes, bioengineering of enzymes for bioactive compounds, the production of hydrolytic and oxidative enzymes from plant raw materials, and prebiotics and probiotics. Given its multidisciplinary scope, this book will appeal to researchers and industry experts in the fields of microbiology, biotechnology and molecular medicine.

The book will benefit a reader with a background in physical sciences and applied mathematics interested in the mathematical models of genetic evolution. In the first chapter, we analyze several thought experiments based on a basic model of stochastic evolution of a single genomic site in the presence of the factors of random mutation, directional natural selection, and random genetic drift. In the second chapter, we present a more advanced theory for a large number of linked loci. In the third chapter, we include the effect of genetic recombination into account and find out the advantage of sexual reproduction for adaptation. These models are useful for the evolution of a broad range of asexual and sexual populations, including virus evolution in a host and a host population.

The book provides the readers of various discipline an easy understanding of the latest biophysical techniques pertaining to microbiology. Biofilm associated chronic infection is a major health problem and a serious concern to doctors, scientists and other health workers as it develops antibiotic and multi-drug resistance. This book describes various protocols utilized in the detection of the biofilm. The book has been divided into six sub sections which provides pertinent information about the various biophysical techniques and instruments that are used for detecting and analyzing the biofilm formation upon biotic and abiotic surfaces. The readers will be able to identify the techniques that can best cater information to solve the problem at hand. This book attempts to compile the latest information on the recent advances in the various functional aspects of microbial biofilms, their pathogenesis, present day treatments as well as detection strategies. This book is meant for researchers in the field of microbiology and interested in understanding microbial pathogenesis, quorum sensing and biofilm formation.