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This book gives an accessible, detailed overview on techniques of
single molecule biophysics (SMB), showing how they are applied to
numerous biological problems associated with understanding the
molecular mechanisms of DNA replication, transcription, and
translation, as well as functioning of molecular machines. It
covers major single molecule imaging and probing techniques,
highlighting key strengths and limitations of each method using
recent examples. The chapters begin with a discussion of single
molecule fluorescence techniques followed by an overview of the
atomic force microscope and its use for direct time-lapse
visualization of dynamics of molecular complexes at the nanoscale,
as well as applications in measurements of interactions between
molecules and mechanical properties of isolated molecules and their
complexes. The next chapters address magnetic tweezers and optical
tweezers, including instrumentation, fundamentals of operation, and
applications. A final chapter turns to nanopore transport and
nanopore-based DNA sequencing technology that will play a major
role in next-generation genomics and healthcare applications.
This book gives an accessible, detailed overview on techniques of
single molecule biophysics (SMB), showing how they are applied to
numerous biological problems associated with understanding the
molecular mechanisms of DNA replication, transcription, and
translation, as well as functioning of molecular machines. It
covers major single molecule imaging and probing techniques,
highlighting key strengths and limitations of each method using
recent examples. The chapters begin with a discussion of single
molecule fluorescence techniques followed by an overview of the
atomic force microscope and its use for direct time-lapse
visualization of dynamics of molecular complexes at the nanoscale,
as well as applications in measurements of interactions between
molecules and mechanical properties of isolated molecules and their
complexes. The next chapters address magnetic tweezers and optical
tweezers, including instrumentation, fundamentals of operation, and
applications. A final chapter turns to nanopore transport and
nanopore-based DNA sequencing technology that will play a major
role in next-generation genomics and healthcare applications.
This volume presents readers with the latest techniques to study
nanoimaging and nanoprobing in application to a broad range of
biological systems. The chapters in this book are divided into five
parts, and cover topics such as imaging and probing of
biomacromolecules including high-speed imaging and probing with
AFM; probing chromatin structure with magnetic tweezers; and
fluorescence correlation spectroscopy on genomic DNA in living
cells. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
through, Nanoscale Imaging: Methods and Protocols is a valuable
resource for anyone interested in learning more about this
developing and expanding field.
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