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Books > Medicine > General issues > Medical equipment & techniques > Medical laboratory testing & techniques
Handbook of Flow Cytometry Methods Edited By J. Paul Robinson
Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn
Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory
manual for investigators involved in research and diagnostic
applications of flow cytometry. Handbook of flow cytometry methods
offers a collection of proven, basic procedures designed for use in
the flow cytometry laboratory. Methods implemented at Purdue
University Cytometry Laboratories and developed in laboratories of
contributors worldwide provide practical, step-by-step procedures
for:
- the preparation and handling of standards, reagents and
specimens
- laboratory analyses for immunophenotyping
- measurement of DNA content and cell cycle analyses
- determination of cell physiology and function
- set up and operation of flow cytometers
- computational and data analysis techniques
- safety precautions
Drawing on their collective experience at leading research centers,
the editors have fashioned a unique "working manual" intended to
serve as the starting point for the evaluation and establishment of
laboratory benchmark standards in flow and image cytometry. Each
method is described in detail to be used and modified depending on
the user's needs. Special features of Handbook of Flow Cytometry
Methods include:
- ample space throughout for personal annotations
- suggestions of alternative procedures; trouble-shooting
tips
- extensive list of reagents and suppliers
- comb-bound format for ease of use at the lab bench
Handbook of Flow Cytometry Methods is an essential laboratory
resource for students, faculty, and researchers in cell and
molecular biology, immunology, cancer biology, hematology/oncology,
and laboratory medicine.
This updated and enlarged second edition is a unique source of
information on the diagnosis, treatment, and follow-up of metabolic
diseases. The clinical and laboratory data characteristic of rare
metabolic conditions can be bewildering for clinicians and
laboratory personnel alike - reference laboratory data is
scattered, and clinical descriptions can be obscure. The new
Physician's Guide with the additional more than 600 diseases now
featured, documents 1200 conditions grouped according to type of
disorder, organ system affected (e.g. liver, kidney, etc) or
phenotype (e.g. neurological, hepatic, etc). It includes relevant
clinical findings and highlights the pathological values for
diagnostic metabolites. Guidance on appropriate biochemical genetic
testing is also provided and established experimental therapeutic
protocols are described, with recommendations on follow-up and
monitoring. The authors are acknowledged experts, and the book is a
valuable desk reference for all who deal with inherited metabolic
diseases. Chapter 73 is available open access under a Creative
Commons Attribution 4.0 International License via link.springer.com
4 Stars from Doody's Book Review (on the Third Edition)! "It is
excellent reading for students, healthcare professionals
considering the cath lab as an adventure, as well as novice and
expert cath lab professionals." Completely revised and updated, the
Fourth Edition of Invasive Cardiology: A Manual for Cath Lab
Personnel, is written specifically for nurses, technologists, and
allied health personnel working in the catheterization laboratory.
Topics cover all aspects of the catheterization laboratory
including cardiovascular anatomy, radiography, angiography,
technical duties of the staff, right and left heart
catheterization, PCI, invasive ultrasound, valvuloplasty,
hemostasis, pediatric interventions, pharmacology, emergency
procedures, and many others. Every new print copy includes Navigate
Advantage Access that unlocks a complete, interactive eBook,
student practice activities, learning analytics reporting tools,
and more! Now focused on being a training and assessment resource
rather than just a reference book, the book is divided into parts
for better pedagogical organization. Each chapter now includes
learning objectives, summaries, relevant case studies, and
self-assessment questions. Updated to reflect the Society of
Invasive Cardiovascular Professionals' (SICP) 2015 Educational
Guidelines for Invasive Cardiovascular Technology Personnel in the
Cardiovascular Catheterization Laboratory. New instructor resources
include slides in PowerPoint and an Instructor Manual with chapter
outlines, online educational resources, and suggested readings.
Includes information on endovascular treatment of pulmonary
embolism. New eBook available with additional online case studies.
Printable step-by-step procedures.
Whilst assisted reproduction techniques (ART) have become
increasingly successful and largely standardized, there is still
only a partial understanding of what constitutes a 'true' embryo
environment. Replicating the varying physiological conditions of
the in-vivo environment that the embryo travels through in the
in-vitro culture is still a major challenge in ART. This practical
volume details how to organize and operate an IVF laboratory in
order to mimic these conditions for successful embryo culture.
Environments and equipment that are essential for running safe and
efficient facilities such as maintaining good air quality and
hygiene protocols, and utilizing an effective layout are covered in
detail. Other chapters discuss the different consumables needed,
optimal handling techniques and parameter monitoring systems, as
well as recent advances in the area including artificial
intelligence and automation. This is an indispensable guide to
understanding the background science of culturing embryos, crucial
to successful outcomes in ART.
Pharmacological biotechnology is applied to and used to study drug
development, working mechanisms, diagnosis, and therapies. This
textbook covers the whole range of experiments related to
pharmacology. It also contains basic laboratory safety guidelines
along with the basic calculations and formulas used in a
laboratory. Each chapter starts with an introduction/theory into
the basic approach followed by detailed methods sections with
easy-to-follow protocols and comprehensive troubleshooting,
calculations and possible questions for examination. The target
group is researchers who are studying pharmacological biotechnology
in the laboratory.
Biomedical scientists are the foundation of modern healthcare, from
cancer screening to diagnosing HIV, from blood transfusion for
surgery to food poisoning and infection control. Without biomedical
scientists, the diagnosis of disease, the evaluation of the
effectiveness of treatment, and research into the causes and cures
of disease would not be possible. The Fundamentals of Biomedical
Science series has been written to reflect the challenges of
practicing biomedical science today. It draws together essential
basic science with insights into laboratory practice to show how an
understanding of the biology of disease is coupled to the
analytical approaches that lead to diagnosis. Assuming only a
minimum of prior knowledge, the series reviews the full range of
disciplines to which a Biomedical Scientist may be exposed-from
microbiology to cytopathology to transfusion science. The science
of transfusion and transplantation demands a multifaceted
understanding of immunology, haematology, and genetics from the
biomedical scientist. Transfusion and Transplantation Science
synthesizes the essential concepts of these subjects and presents
them within the practical framework of the hospital banking and
transplantation centre, providing you with the knowledge and skills
to specialize in this discipline.
This book highlights the increase in thyroid tumors and NET and
demonstrates the growing importance of circulating markers in
diagnosis as well as treatment and follow-up. Dramatic technical
improvements have heightened the clinical impact of
well-established, conventional biochemical markers. In addition,
more recent genetic and molecular approaches have provided
innovative molecular markers. In this context, effective
communication between clinicians and laboratory
physicians/scientists is essential in allowing all those involved
to fully profit from these exciting advances. In this
comprehensive, up-to-date book, authors from different laboratory
and clinical areas link laboratory and clinical topics. Analytical
problems such as interferences, false-negative and false-positive
results are discussed in depth, and flow-charts offer insights into
identifying and avoiding them. Illustrated clinical cases detail
the clinical role and limitations of different tumor markers.
Lastly, it explores health technology assessment and economic
issues. This is a valuable resource for endocrinologists,
oncologists, nuclear medicine physicians, scientists and
technologists who want to keep abreast of the latest developments.
Pathology is an inherently subjective discipline, and therefore is
often referred to as both an art and a science. Over the years,
laboratory physicians have implemented special tissue stains and
molecular techniques to limit subjectivity in the discipline.
Beginning in the late 19th century, histochemical stains were
developed to assess diagnostic biochemical reactions in tissue.
Histochemistry has recently seen a resurgence in popularity because
of the higher costs of other newer methods. Today, this technique
is used by almost every pathology laboratory across the world.
This book comprehensively covers all diseases for which that
technique plays a central role in diagnosis. Every anatomic region
is covered in detail with examples of appropriate staining
techniques, and the book is heavily illustrated with over 850 color
photomicrographs. This is the first monograph to be published on
histochemistry in 15 years, and it is the only one that is
diagnostically-oriented.
Emphasizing the relevance of microbiology to a career in the health
professions, Burton's Microbiology for the Health Sciences provides
the vital microbiology information you need to protect yourself and
your patients from infectious diseases.
A Davis’s Notes book! This pocket-sized reference provides great
information on phlebotomy techniques, with nice summaries of
procedures with many photos and illustrations. It is ideal for
clinical rotations, for quick review of coursework, and to study in
preparation for your certification exam. The write-on/wipe-off
pages feature a dedicated space where you can fill in the specific
requirements for evacuated tubes, instrumentation, and analytical
procedures for two different locations. Clinically focused, it’s
the perfect guide to collecting, transporting, and processing
quality blood specimens for laboratory testing. Essential Pocket
Guide for Phlebotomy! "It has a lot of information, with
step-by-step instructions with pictures. This is essential if you
will be doing any phlebotomy of any kind." - Pamela O., Amazon
Reviewer Great resource. "Love this book! While doing my externship
this book was recommended to me. I am very glad that I ordered it.
It includes labs and the corresponding tube colors which is
something I needed. It is wipeable and will fit in the pocket of my
scrubs. I have already recommended it to the other students who
were in class with me." - T.S, Amazon Reviewer Phlebotomist Must
Have! "These Phlebotomy Notes were very helpful overviewing all key
notes for my Exam and while doing my Clinicals, very easy to
navigate. Will continue to use it through my Career in Phlebotomy."
- Cali, Amazon Reviewer
Graff's Textbook of Urinalysis and Body Fluids, Third Edition
features short, easy-to-digest chapters, and an extensive array of
built-in study aids to help you master key content.
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