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The Plant Plasma Membrane - Structure, Function and Molecular Biology (Paperback, Softcover reprint of the original 1st ed. 1990)
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The Plant Plasma Membrane - Structure, Function and Molecular Biology (Paperback, Softcover reprint of the original 1st ed. 1990)
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The plasma membrane forms the living barrier between the cell and
its surroundings. For this reason it has a wide range of important
functions related to the regulation of the composition of the cell
interior and to com munication with the cell exterior. The plasma
membrane has therefore attracted a lot of research interest. Until
the early 1970's it was only pos sible to study the plasma membrane
in situ, its structure e. g. by electron microscopy and its
function e. g. by uptake of radioactively labeled com pounds into
the intact cell or tissue. The first isolation of plant protoplasts
by enzymatic digestion of the cell wall in the early 1970's was an
important step forward in that it provided direct access to the
outer surface of the plasma membrane. More importantly, T. K.
Hodges and R. J. Leonard in 1972 published the description of a
method by which a fraction enriched in plasma membranes could be
isolated from plant tissues using sucrose gradient centrifugation.
As a result, the 1970's saw a leap forward in our understanding of
the structurc and function of the plasma membrane. In 1981, S.
Widell and C. Larsson published the first of a series of papers in
which plasma membrane vesicles of high yield and purity were
isolated from a wide range of plant tissues using aqueous polymer
two-phase parti tioning.
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