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Methods in Membrane Biology - Volume 6 (Paperback, Softcover reprint of the original 1st ed. 1976)
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Methods in Membrane Biology - Volume 6 (Paperback, Softcover reprint of the original 1st ed. 1976)
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Less than a year before this writing, a Nobel Prize was shared by
Albert Claude, Christian de Duve, and George Palade, pioneers in
the development of modern cell biology, of which membrane biology
is an integral part. For many years, a seemingly unbridgeable gap
separated the physiologist working at the organ level from the
biochemist studying the molecular composition of cell constituents
and the chemical reactions that occur in water-soluble extracts of
cells. Physiology has a long history, and the disciplines
epitomized by intermediary metabolism and molecular biology
progressed rapidly during the 1950s and 1960s. Meanwhile, electron
micros copists painstakingly mapped the newly discovered
intracellular world of membranes, organelles, microtubules, and
microfilaments, and other scien tists developed techniques for the
quantitative separation and characteriza tion of these
intracellular structures. Thus it finally became possible to
localize the many enzymes, and the metabolic activities they
catalyze, to recognizable structures whose composition and
organization can be studied. We are now well on our way to bridging
that gap between biochemistry and physiology-to understanding how
the cell functions.
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