Contents References 11 II. A short historical survey 13 References
16 III. Ribosomes within the cell 20 1. Proportion of ribosomes in
tissues 21 2. Free and membrane-bound ribo- mes 22 3. Ribosomes in
nucleoli 26 4. Structure of polysomes in the cell 26 5. Ribosomal
crystals 33 6. References 33 IV. Some general properties of
ribosomes 41 1. Physical characteristics 41 2. Chemical
characteristics 43 3. References 44 V. Morphology of ribosomes and
poly- somes 47 1. Electron microscopy 47 a) The small ribosomal
subunit 48 b) The large ribosomal subunit 48 c) The monomeric
ribosome 52 d) Three-dimensional models 54 e) Polysomes 55 2.
Small-angle X-ray scattering 55 a) The monomeric ribosome 56 b)
Polysomes 57 3. References 57 VI. Chemical components 61 1.
Ribosomal proteins 61 a) Electrophoretic separation and num- ber 61
b) Preparation of single ribosomal pro- teins 67 c) Molecular
weights 68 d) Amino acid composition and amino acid sequences 71 e)
Stoichiometry 71 f) Posttranslational modifications 72 g)
Comparison of ribosomal proteins of different tissues 73 h) Species
specificities and evolution 74 i) Ribosomal proteins of
mitochondria and chloroplasts 75 k) Ribosomal mutants with altered
pro- teins 75 5 3. Formation of preribosomal 2. Phosphorylation of
ribosomal prote- particles 139 ins 76 4. References 141 a) In vitro
phosphorylation and de- phosphorylation 76 VIII. Dissociation -
reassociation processes b) In vivo phosphorylation 77 of ribosomal
particles 151 c) Phosphorylation of ribosomal pro- tein S 6 78 1.
General
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