This volume covers protocols related to both pluripotent and
somatic stem cells, including the ethical procurement of tissues
and cells for the provision of "seed stock," standardized methods
for deriving hESCs and iPSCs, isolating mesenchymal stem cells,
cell culture and cryopreservation, in addition to quality assurance
and information management. Stem Cell Banking: Concepts and
Protocols aims to contribute to the development of this field by
providing information that is essential to establishing a bona fide
stem cell bank. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Stem Cell Banking: Concepts and
Protocols is a valuable resource for stem cell scientists and
novices to the field, and will help strengthen and maximize their
use of existing and future stem cell resources.
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