Taro leaf blight caused by Phytophthora colocasiae is a major
constraint to taro cultivation. To manage taro leaf blight and
design breeding programme, it is crucial to recognize the genetic
diversity of taro and P. colocasiae. We studied genetic structure
of taro and P. colocasiae in India. Biochemical alterations in taro
plants infected by the P. colocasiae were studied and changes in
peroxidase, -1,3-glucanase, L-phenylalanine ammonia-lyase, total
phenol and total sugar were evaluated. We employed suppressive
subtractive hybridization, high through put DNA sequencing and
bioinformatics to identify the defense related genes in taro
induced by P. colocasiae infection. Two putative resistance genes
and a transcription factor were identified among the upregulated
sequences. Our study illustrated that identified genes will be
useful to engineer disease protection in taro."
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