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The Baculovirus Expression System - A laboratory guide (Paperback, Softcover reprint of the original 1st ed. 1992)
Loot Price: R2,342
Discovery Miles 23 420
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The Baculovirus Expression System - A laboratory guide (Paperback, Softcover reprint of the original 1st ed. 1992)
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The decision to write a book about the practical aspects of the
baculovirus expression system stems from the numerous phone calls
for help we have had, and from the many visitors to our labora
tories requiring assistance to find the elusive polyhedrin-negative
virus containing their favourite gene. We have also organized two
expression system workshops and from the manuals we wrote for
these, it seemed a logical progression to extend them into book
form. We appreciate that those who are 'old-hands' at the
baculovirus expression system may have differing views on some of
our procedures, but the methods in this book are presented in the
light of our own experiences in the laboratory and from our
practical workshops, and we hope that the book will be especially
useful to those new to the system. The first three chapters give
the background information to the baculovirus expression system,
and includes advice on how to choose the right transfer vector and
discusses the various methods that are available to select
recombinant viruses. The practical chapters concentrate on those
aspects which are novel to the baculovirus system (insect cell
culture, virus amplification and titration, etc. ) and, in general,
leave the standard molecular biological techniques to the other
excellent laboratory manuals that are available. However, for
completeness sake and to avoid constant reference to other manuals,
we have included brief details of some standard techniques where
they are integral to the success of the baculovirus protocols."
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