the discovery of the "splicing" of the gene transcripts, the list
would include the whole molecular genetics of the lambda
bacteriophage, the notions of "promotor," "repressor," and
"integration," the discovery of the reverse flow of genetic
information, the very existence of oncogenes, the S'-terminal "cap"
struc ture of eukaryotic mRNAs, ... Electronmicroscopy,
ultracentrifugation and tissue culture were the landmarks on the
way of the young science. During the past few years, however, a
major (and not so silent) revolution took place: recombinant DNA
technology with all its might entered in our laboratories, and
restriction mapping of cloned genomes and sequencing gels have
replaced plaque counting and sucrose gradients. The new techniques
have made it possible to "dissect" the entire genome of a virus at
the molecular level, and studies that would have been dreamt of
just in the mid-seventies became the everyday experiments of our
days. With new insight into the structure of viral genomes, and a
deeper understanding of the mechanisms that regulate their
expression, our view of viruses was bound to change: this volume
bears witness to this impressive advance."
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