Human cell culture is not a new topic, but the development of new
molecular techniques and reagents which can be used to investigate
cell function and the responsible intracellular mechanisms make it
a continuing requirement. This third edition of Human Cell Culture
Protocols expands upon the previous editions with current, detailed
protocols for the isolation and culture of a range of primary cells
from human tissues. With new chapters on pancreatic cells needed
for basic studies on the pathogenesis of diabetes and for their
application for islet transplantation, the book also delves into
protocols for hepatocytes, skin cells, lung cells, parathyroid
cells, gastric cells, renal cells, adipocytes, ovarian cells, bone
cells, vascular smooth muscle cells, vascular endothelial cells,
regulatory T cells, blood mononuclear cells, as well as new
techniques being applied to human cell culture, particularly the
use of biocompatible scaffolds to grow cells, the in vitro use of
laser microdissection to isolate cells from culture, and automated
cell culture. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, Human Cell Culture Protocols, Third Edition makes it
possible for a worker with basic cell culture training, whether in
the fields of cell biology, gene therapy, and cell transplantation,
to prepare cell cultures of the specific cell type necessary to
forward their vital research.
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