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Human Cell Culture Protocols (Paperback, Softcover reprint of the original 3rd ed. 2012) Loot Price: R3,225
Discovery Miles 32 250
Human Cell Culture Protocols (Paperback, Softcover reprint of the original 3rd ed. 2012): Ragai R. Mitry, Robin D. Hughes

Human Cell Culture Protocols (Paperback, Softcover reprint of the original 3rd ed. 2012)

Ragai R. Mitry, Robin D. Hughes

Series: Methods in Molecular Biology, 806

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Loot Price R3,225 Discovery Miles 32 250 | Repayment Terms: R302 pm x 12*

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Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.

General

Imprint: HumanaPress
Country of origin: United States
Series: Methods in Molecular Biology, 806
Release date: August 2016
First published: 2012
Editors: Ragai R. Mitry • Robin D. Hughes
Dimensions: 254 x 178mm (L x W)
Format: Paperback
Pages: 435
Edition: Softcover reprint of the original 3rd ed. 2012
ISBN-13: 978-1-4939-6208-2
Categories: Books > Science & Mathematics > Biology, life sciences > Cellular biology > General
LSN: 1-4939-6208-6
Barcode: 9781493962082

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