This volume reviews the increasing importance of glycosylation to
the field of virology, as well as virus replication. The chapters
provide an overview of glycosylation in relation to virus
infection, and the generic techniques that are used to analyze and
characterize glycoproteins.
Among the topics discussed in this volume are M.E. interaction
between respiratory syncytial virus and glycosaminoglycans, acute
respiratory syndrome coronavirus 3a protein, the C type lectins
DC-SIGN and L-SIGN, N-linked glycans, and the use of
two-dimensional SDS-PAGE to analyze the glycosylation heterogeneity
of the respiratory syncytial virus fusion protein. Also covered in
this volume are uses of monoclonal antibodies and lectins, the
spike glycoprotein, secretion of respiratory syncytial vius F
protein from insect cells, the expression and characterization of
recombinant dengue virus E protein, and cloning, expression, and a
functional analysis of patient-derived hepatitis C virus
glycoproteins.
The information presented in Glycovirology Protocols will
provide insight as to how the techniques of glycobiology can be
applied in virology and answer most questions that are of interest
to the reader.
General
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