The objective of this research was to develop guidance for
collecting samples of biosolids for microbial analysis to ensure
representative samples are tested. The types of biosolids products
studied included liquid, cake and compost. To accomplish the
research objective, three phases of research and development of a
suite of communications documents were undertaken. The first Phase
involved information gathering and establishing the status of
sampling guidance and practices for biosolids. Phase II involved
conducting sampling and microbial analysis of biosolids products
from four target facilities utilizing different biosolids treatment
technologies to determine which of a series of sample collection
and handling parameters most affects sample integrity and
representativeness. Phase III of the project involved field testing
at nine utilities. Microbial monitoring results were compared and
utility protocols were examined to determine the suitability of
their sampling approach. Finally, a series of communications
documents were prepared. These communications tools were designed
to convey the importance of sampling and handling details at
multiple stakeholder levels. This research demonstrated that
analysis of multiple, discrete, grab samples provides insight into
product variability. In addition, proper handling and adherence to
sample size and storage protocols provides a reliable measurement
of biosolids microbial content from the biosolids production
process being sampled
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