Clinical Applications of Capillary Electrophoresis (Paperback, Softcover reprint of the original 1st ed. 1999)

The term "electrophoresis" was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations-zone, IEF, and isotachophoresis-have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Medicine, 27
Release date:	August 2014
First published:	1999
Editors:	Stephen M. Palfrey
Dimensions:	229 x 152 x 12mm (L x W x T)
Format:	Paperback
Pages:	212
Edition:	Softcover reprint of the original 1st ed. 1999
ISBN-13:	978-1-4899-4174-9
Categories:	Books > Medicine > Other branches of medicine > Pathology > Gross pathology
LSN:	1-4899-4174-6
Barcode:	9781489941749

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