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Gram Stain - Looking Beyond Bacteria to Find Fungi in Gram Stained Smear:A Laboratory Guide for Medical Microbiology (Paperback)
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Gram Stain - Looking Beyond Bacteria to Find Fungi in Gram Stained Smear:A Laboratory Guide for Medical Microbiology (Paperback)
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There are several medical mycology textbooks that contain a chapter
on direct microscopy. However, this textbook is the first of its
kind, as it discusses the simple Gram stain procedure as a valuable
tool for the detection of fungal elements. This book has been
specifically designed for people working in the medical
microbiology laboratory with little or no practical experience in
medical mycology. The central idea presented in this textbook
begins with the Gram stain for the detection of fungi; the most
important and more frequently isolated opportunistic and
potentially pathogenic fungal species have been included. The book
contains more than three hundred color images, the majority of
which come from direct smear examination, such as Gram stain and
other staining procedures. The mold phase and the microscopic
structure of the identified fungal species relating to the initial
findings of the direct smear have been linked to avoid bias. When a
fungal infection is present but not suspected clinically, the Gram
stain may be the only clue to the true cause of the infection.
Although there are better methods than the Gram stain for
visualization of fungi, these methods are only performed if there
is clinical suspicion for fungal disease. Clinicians often send
specimens for bacterial culture, but they sometimes overlook
requests for fungal culture. During such times, the Gram stain is
the only technique available in the clinical microbiology
laboratory for direct detection of fungi from these specimens. The
presence of fungi should not be overlooked during the direct
examination of the clinical specimens for bacteria. This book will
guide the reader in the recognition and identification of fungal
elements in gram-stained smears, especially when they are distorted
and remain unstained and undetectable. This new textbook focuses on
the detection and classification of fungal elements in Gram stains.
Newly developed flowcharts, clues, and key details regarding
structural characteristics have been added to guide the reader in
the right direction. Throughout the years, the author has
accumulated many scenarios in which fungal elements were not
detected on the original Gram stain evaluation but were found to be
positive upon review once the culture grew a fungus. Finally, the
book contains a chapter with a practice examination including
microscopic images representative of scenarios commonly encountered
in the clinical microbiology laboratory.
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