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Performance Assessment and Enrichment of Anaerobic Methane Oxidizing Microbial Communities from Marine Sediments in Bioreactors (Paperback)
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Performance Assessment and Enrichment of Anaerobic Methane Oxidizing Microbial Communities from Marine Sediments in Bioreactors (Paperback)
Series: IHE Delft PhD Thesis Series
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Anaerobic oxidation of methane (AOM) coupled to sulfate reduction
(AOM-SR) is a biological process mediated by anaerobic
methanotrophs (ANME) and sulfate reducing bacteria. It has
scientifi c and societal relevance in regulating the global carbon
cycle and biotechnological application for treating sulfate-rich
wastewater. This research aimed to enhance the recent knowledge on
ANME distribution and its enrichment in different bioreactor confi
gurations, i.e. membrane bioreactor (MBR), biotrickling fi lter
(BTF) and high pressure bioreactor (HPB). Marine sediment from
Ginsburg mud volcano, Gulf of Cadiz was used as inoculum in the BTF
and MBR. The BTF operation showed the enrichment of ANME in the
biofi lm, especially ANME-1 (40%) and ANME-2 (10%). Whereas, the
dominancy of ANME-2 and Desulfosarcina aggregates was observed in
the MBR. Moreover, HPB study was performed by using highly enriched
ANME-2 community from Captain Arutyunov mud volcano. During the
study of HPB at different temperature and pressure conditions, the
incubation at 10 MPa pressure and 15 C was observed to be the most
suitable condition for the studied AOM-SR community. Furthermore,
AOM-SR activity in the coastal sediments from marine Lake
Grevelingen (the Netherlands) was explored and the microbial
community was characterised which was dominated by ANME-3 among
known ANME types.
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