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Protein glycosylation is now acknowledged as a major
posttranslational modification with significant effects on protein
folding, conformation distri- bution, stability, and activity. The
added oligosaccharide chains are large and diverse and have
specific recognition motifs important in many aspects of cell
interactions and regulation. As such, there is a growing need to
communicate the analytical methods of the specialist carbohydrate
chemist, biochemist, and physicochemist to protein experts and the
pharmaceutical industry. Other areas that come under the influence
of the glycosciences are DNA interactions with ubiquitous
saccharide-containing antibiotics and antitumor drugs; inhibitors
of viral infection; bacterial, mycobacterial, and parasite
antigens; glycolipids; glycophosphatidylinositol protein membrane
anchors; and (glyco)protein- proteoglycan interactions. Compared to
the first edition of this book, Glycopro- tein Analysis in
Biomedicine, less emphasis is given to biomedical aspects, but
these chapters are still pertinent today. The significant
differences in the con- tent relate to advances in analysis
relevant to biotechnology; for example, the production of
recombinant glycoproteins and other therapeutics. It must also not
be forgotten that the methods here described in Glycoanalysis
Protocols are relevant to exploiting the commercial potential of
carbohydrates in fields related to agriculture, food, and the
domestic and chemical industries. The emphasis of the book remains
in bringing the glycosciences into mainstream biochemistry. The
analytical methods covered in Glycoanalysis Protocols are the re-
sult of experts translating their life's works into easy-to-follow
recipes.
Protein glycosylation is now acknowledged as a major
posttranslational modification with significant effects on protein
folding, conformation distri- bution, stability, and activity. The
added oligosaccharide chains are large and diverse and have
specific recognition motifs important in many aspects of cell
interactions and regulation. As such, there is a growing need to
communicate the analytical methods of the specialist carbohydrate
chemist, biochemist, and physicochemist to protein experts and the
pharmaceutical industry. Other areas that come under the influence
of the glycosciences are DNA interactions with ubiquitous
saccharide-containing antibiotics and antitumor drugs; inhibitors
of viral infection; bacterial, mycobacterial, and parasite
antigens; glycolipids; glycophosphatidylinositol protein membrane
anchors; and (glyco)protein- proteoglycan interactions. Compared to
the first edition of this book, Glycopro- tein Analysis in
Biomedicine, less emphasis is given to biomedical aspects, but
these chapters are still pertinent today. The significant
differences in the con- tent relate to advances in analysis
relevant to biotechnology; for example, the production of
recombinant glycoproteins and other therapeutics. It must also not
be forgotten that the methods here described in Glycoanalysis
Protocols are relevant to exploiting the commercial potential of
carbohydrates in fields related to agriculture, food, and the
domestic and chemical industries. The emphasis of the book remains
in bringing the glycosciences into mainstream biochemistry. The
analytical methods covered in Glycoanalysis Protocols are the re-
sult of experts translating their life's works into easy-to-follow
recipes.
As the editorof Glycoprotein Analysis in BiornedidneI have tried to
bring together a comprehensive range ofdetailed protocols that will
rendercarbohydratebiochemistryaccessible to all, andnotjust to the
dedicated few. I wish to thank the authors, all ofwhom have striven
significantly to realize this aim. There is now a growing interest
in glycoprotein analysis from researchers in many disciplineswho
realize thatprotein glycosylation can no longer be ignored.
Oligosaccharide chains of glycoproteins form a diverse
arrayofmolecules with an inherentlylarge contentof chemical
information that is functional in biological recognition. In
addition to theirspecificinteractionswithantibodiesandcarbohydrate
binding proteins, the carbohydrate moieties of glycoproteins have
profoundly important effects on the conformations, stability,
antige nicity, and function ofthe protein components. Some
specialized techniques, Le., NMRand MS, are introduced
atthebeginningofthebooksince thesearecapableofdefiningexactly the
diversityofoligosaccharide sequences, and are used to detect the
growing number ofnew structures as well as to indentiry, in new sce
narios, those previously documented. Together with the chromato
graphic methods of purification and analysis discussed in Chapters
1-12, there now exists a powerful armamentarium for glycoprotein
characterization. The more chemical techniques are documented in
detail in the earliest chapters, whereas the enzymatic methods of
releasing oligosaccharidesfrom protein areintroducedlater. Analysis
ofglycoproteinsfrom mammalian, parasitic, bacterial,
andviralorigins are each discussed."
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