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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Glycoprotein Analysis in Biomedicine (Paperback, 1993 ed.)
Loot Price: R2,896
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Glycoprotein Analysis in Biomedicine (Paperback, 1993 ed.)
Series: Methods in Molecular Biology, 14
Expected to ship within 10 - 15 working days
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As the editorof Glycoprotein Analysis in BiornedidneI have tried to
bring together a comprehensive range ofdetailed protocols that will
rendercarbohydratebiochemistryaccessible to all, andnotjust to the
dedicated few. I wish to thank the authors, all ofwhom have striven
significantly to realize this aim. There is now a growing interest
in glycoprotein analysis from researchers in many disciplineswho
realize thatprotein glycosylation can no longer be ignored.
Oligosaccharide chains of glycoproteins form a diverse
arrayofmolecules with an inherentlylarge contentof chemical
information that is functional in biological recognition. In
addition to theirspecificinteractionswithantibodiesandcarbohydrate
binding proteins, the carbohydrate moieties of glycoproteins have
profoundly important effects on the conformations, stability,
antige nicity, and function ofthe protein components. Some
specialized techniques, Le., NMRand MS, are introduced
atthebeginningofthebooksince thesearecapableofdefiningexactly the
diversityofoligosaccharide sequences, and are used to detect the
growing number ofnew structures as well as to indentiry, in new sce
narios, those previously documented. Together with the chromato
graphic methods of purification and analysis discussed in Chapters
1-12, there now exists a powerful armamentarium for glycoprotein
characterization. The more chemical techniques are documented in
detail in the earliest chapters, whereas the enzymatic methods of
releasing oligosaccharidesfrom protein areintroducedlater. Analysis
ofglycoproteinsfrom mammalian, parasitic, bacterial,
andviralorigins are each discussed."
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