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Glycoanalysis Protocols (Hardcover, 2nd ed. 1998)
Loot Price: R3,237
Discovery Miles 32 370
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Glycoanalysis Protocols (Hardcover, 2nd ed. 1998)
Series: Methods in Molecular Biology, 76
Expected to ship within 12 - 17 working days
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Protein glycosylation is now acknowledged as a major
posttranslational modification with significant effects on protein
folding, conformation distri- bution, stability, and activity. The
added oligosaccharide chains are large and diverse and have
specific recognition motifs important in many aspects of cell
interactions and regulation. As such, there is a growing need to
communicate the analytical methods of the specialist carbohydrate
chemist, biochemist, and physicochemist to protein experts and the
pharmaceutical industry. Other areas that come under the influence
of the glycosciences are DNA interactions with ubiquitous
saccharide-containing antibiotics and antitumor drugs; inhibitors
of viral infection; bacterial, mycobacterial, and parasite
antigens; glycolipids; glycophosphatidylinositol protein membrane
anchors; and (glyco)protein- proteoglycan interactions. Compared to
the first edition of this book, Glycopro- tein Analysis in
Biomedicine, less emphasis is given to biomedical aspects, but
these chapters are still pertinent today. The significant
differences in the con- tent relate to advances in analysis
relevant to biotechnology; for example, the production of
recombinant glycoproteins and other therapeutics. It must also not
be forgotten that the methods here described in Glycoanalysis
Protocols are relevant to exploiting the commercial potential of
carbohydrates in fields related to agriculture, food, and the
domestic and chemical industries. The emphasis of the book remains
in bringing the glycosciences into mainstream biochemistry. The
analytical methods covered in Glycoanalysis Protocols are the re-
sult of experts translating their life's works into easy-to-follow
recipes.
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