The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols."

Despite over fifty years of literacy training by the Mexican government, the National Census records an illiteracy rate of over 70 percent in most Indian communities. This book attempts to discover why so many Indians are illiterate today despite an indigenous literary tradition that dates back to the pre-Conquest period. The author sees language as the main factor explaining the high illiteracy rate in the Indian regions. Although alphabets have been created for most of Mexico's indigenous languages, there is no longer a literate tradition in the languages themselves, and writing is intrinsically associated with the official and dominant language, Spanish. Indians continue to reproduce their group identity through the maintenance of linguistic and cultural boundaries. How these boundaries have been built over time and how they continue to be maintained throughout the twentieth century form the substance of this book.

This special issue of the International Review of Education includes contri butions on indigenous knowledge, the cultural context of learning and on the interplay between the so-called "traditional" and "modern" ways of educa tion. It starts from the assumption that cultures are not static, that they are shifting and mutating, and that the Western need to encapsulate "other cultures," which found its most extreme form in their being frozen in time and boxed behind glass in museums of ethnology, has distorted our under standing of the way in which different cultures create, recreate and repro duce knowledge. The basic premise of this position is that there is no such thing as a pure culture, and that all cultures borrow, lend, adapt, and distort distinct elements from other cultures. All cultures, moreover, provide their members with ways of learning about that culture, which include elements such as language, forms of social organisation, and ritual spaces for the trans mission of specialised knowledge. Meaning may be shifted over time, but that in itself is a product of the passage of knowledge through history. Indeed, much meaning is cyclical and reinterpretive so that cultures may look back to a mythological past which they assumed gave them their essential identity but which may be part fact, part fantasy, and part fiction. This is then rein terpreted in the light of changed and changing historical circumstances."

Every Season is the Season for Peace ?Thoughts of Peace? assures the reader that peace is God's plan for everyone who will simply pursue it. It then explains practical ways of pursuing God's peace and the enormous blessings resulting from finding ?a place of peace.? If you desire an abiding peace--not just a fleeting or momentary peace, but one which is an anchor to the soul, steadying you and safely guiding you through every storm in life, then ?Thoughts of Peace? should be the next book on your reading list.

Throughout the series, "Leaving, Cleaving and Loving...for life," the authors explore ways couples can enjoy exciting, loving, lifelong marriages. The series examines how marital unions can be established and maintained to meet, complete and "fill to the full" the design and desire of the Architect and Builder of marriage in every measure. This invokes God's delight and blessing, and allows couples to discover and realize where they fit within this masterpiece called "marriage" and to experience the most purposeful and fulfilling lives possible.

Throughout the series, ?Leaving, Cleaving and Loving...for life, ? the authors explore ways couples can enjoy exciting, loving, lifelong marriages. The series examines how marital unions can be established and maintained to meet, complete and ?fill to the full? the design and desire of the Architect and Builder of marriage in every measure. This invokes God's delight and blessing, and allows couples to discover and realize where they fit within this masterpiece called ?marriage? and to experience the most purposeful and fulfilling lives possible

"Heaven in a Hurry" is the companion to our book, "Confessing God's Best for Your Family." "Heaven in a Hurry" teaches us to boldly confess God's Word in all of life's circumstances and to summon all the power and resources of heaven on our behalf. Speaking God's Word into the atmosphere attracts God's attention towards us and our needs. It also trains us to proclaim and pray God's Word over our families and speak life to our households with the full confidence that, because God hears us, we have the answers to the petitions which we have placed before Him.

Building Blocks for Creating a New Life... A Transformational Journey is a dynamic collaboration and presentation of Universal Knowledge and Principles that takes you on a step by step journey toward self discovery. Through the use of creative visualization, journel exercises, prayer, meditation and affirmations the journey helps the reader discover and sort out exactly what they desire in life, and then provides directions and guidance towards those exact desires and dreams. The book enables you to achieve higher virtues by experiencing better Love, Peace, Clarity, Prosperity, Health, Forgiveness, Freedom of Fear, Overcoming Loss and much more. Beautiful visualizations are presented through meditations, and prayers, that assist in transforming, recreating, and experiencing life in a new way. Building Blocks for Creating a New Life... A Transformational Journey is your guide to creating your New Life

"The Joy of Getting It Done" is a step-by-step workbook for people who want to stop wasting time and start managing it. Life Coach and ADHD Coach Linda King presents 10 simple and realistic tools to help you get your life under control. In-depth exercises will help you introduce new routines, tackle overwhelming projects, and face tasks that you regularly avoid, creating a realistic plan for productivity.

You'll learn how to: Break It Down: A new approach to organizing your space so you see results fast. Avoid Personal Black Holes: Conquer the time-wasters that sabotage your best intentions. Start Small: Begin an exercise program with as little as one minute a day Add to a Routine: Build on the basic routines you already have. Use a Whole Number: Use a simple kitchen timer to overcome procrastination. And more

Are parts of your personal or professional life spiraling out of control? Are you overwhelmed by clutter and paper? Do you waste too many hours in front of electronic devices? Do you start diet or exercise programs with a bang, then fizzle out? Are you perpetually late? Do you avoid big projects because you don't know where to begin?

If you can relate to any of these, "The Joy of Getting It Done" will help you get back on track. The easy-to-follow workbook format makes it possible for even the most overwhelmed person to succeed with Linda King's program. Real examples, many from Linda's own life and work with clients, illustrate how a series of simple, basic steps can lead you to permanent, productive change.

Linda King's "Dream Street Details" identifies the storyteller's struggle for the word, trapped in a netherworld like some minor character with few reference points, learning as they go, filled with wonder, terror, and everything in between. As one forever sewn to their past, especially their childhood, the consistent narrator of the poems certifies that "language does such gentle harm in its steel needle stitching of stories." The first section of the book entitled "some bones fit the page" consists of previously published work, paying homage to the somewhat subdued success the writing life brings and serves as an introduction to the second, main section: "the clues are everywhere," which takes the reader on a journey searching for answers in the elements of language, time and place.