Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, with the focus on the most advanced cutting-edge breakthroughs, covers all the essential aspects of the field of molecular farming in plants: from expression technologies to downstream processing, from products to safety issues, and from current advances and holdups to future developments.

During the 45 years of communist regimes in Hungary, Poland and Slovakia, agriculture was centrally directed without regard of quality factors and market needs, and was heavily subsidized. Democratization of the countries and adoption of market driven economies, including agriculture, created conditions that require new thinking and approaches to find market areas that will fill the needs of each country, and provide produce that is not redundant for the Common Market. The aim of this book is to facilitate solving common agricultural problems using the tools of biotechnology. The book addresses five themes: Plant Transformations, Plant Genomics, Breeding Plants for Resistance and Legal Aspects of Biotechnology, including risk assessment. Some specific topics dealt with are: The way from fundamental research to start-up company, Crop improvement by transgenic technology; Strategies for improving resistance; Current procedures for applying risk assessment in genetically modified crops; Questions arising from the implementation of the Hungarian gene technology law; Public perception and legislation of Biotechnology in Poland.

This volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies.

Predictive control is a powerful tool in dealing with those processes with large time delays. Generalized Predictive Control (GPC) is the most popular approach to the subject, and this text discusses the application of GPC starting with the concept of long-range predictive control and its need in medicine (particularly automated drug deliveries). The concept of adaptation is also emphasized with respect to patient-to-patient parameter variations. Subsequent chapters discuss interactions, comparisons and various aspects of GPC. The book concludes by putting into perpective the generic nature of the architecture built around GPC and which provides model-based fault diagnosis with control.

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as -plasmid biology -nucleic acid biochemistry -restriction enzymes -cloning strategies -gel electrophoresis -southern and northern blotting -preparation of probes -phage lambda biology -cosmids and genome analysis -cloned gene expression -polymerase chain reaction -conventional and automated DNA sequencing -site-directed mutagenesis -and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

This thesis presents original research on how to seamlessly integrate electronics with living biological systems. Jia Liu has used silicon nanowires as active sensors to investigate biological signals at the cellular level. He has also designed nanoelectronic networks into flexible, three-dimensional (3D) and macroporous architectures, which mimic the structure of tissue scaffolds for in vitro 3D integrations with synthetic tissues and in vivo implantation by means of syringe injection. Importantly, the results demonstrate 3D interpenetrations of nanoelectronic networks with neural networks, 3D mapping of tissue activity and long-term implantation with minimal immunoresponses. Further, the book discusses potential applications for pharmacological studies, brain activity mapping and nanoelectronics enabled therapies. The findings presented here have gained wide recognition, including a top research ranking by Chemical & Engineering News and being listed among Scientific American's 10 world changing ideas in 2015.

All the information necessary to set up and run a tissue culture facility is provided in this introductory book.; ; Includes an overview of all the basic tissue culture techniques and describes in detail both the theoretical background and the practical a

Plant protoplasts have proved to be an excellent tool for in vitro manipu- lations, somatic hybridization, DNA uptake and genetic transformation, and for the induction of somaclonal variation. These studies reflect the far- reaching impact of protoplast alterations for agriculture and forest bio- technology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the litera- ture, focusing on recent information and the state of the art in protoplast Plant Protoplasts manipulation and genetic transformation. This book, and Genetic Engineering VI, like the previous five volumes published in 1989,1993, and 1994, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Arachis, Bupleurum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physallis, Platanus, Prunus, Saposhnikovia, Solanum, Spinacia, Trititrigia, Tulipa, and Vaccinium; including fruits such as apricot, banana, cranberry, pepino, peach, and plum. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general bio- technology. New Delhi, August 1995 Professor Y. P. S. BAJA] Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plants from Protop1asts of Arachis Species (Peanut) Z. LI, R. L. JARRET, and J. W. DEMSKI (With 2 Figures) 1 Introduction ...3 2 Isolation of Pro top lasts ...4 3 Culture of Protoplasts ...

Statistical, Mapping and Digital Approaches in Healthcare addresses all health territories, starting from the analysis of geographical data (health data, population data, health data systems and environmental data), to new health areas (Health 3.0), i.e. digital health territories. Specific tools are used to question environmental changes, such as health statistics, mapping, mathematical models, optimization models and serious games.

Computerized machines can be found in many forms and all around us - in our pockets, and even sometimes in our body. For many of us, they are now essential elements of everyday life. When it comes to smartphones, connected objects, medical digital devices and e-health, these digital tools have proliferated in our environment, continually transforming our modes of social organization. They act as prostheses and orthotics that "enhance" our cognitive capacities and influence our inherent behaviors. Are digital tools that perpetually envelop the body and the spirit able to overwhelm the social order? Could our cognitive prosthetics lead to permanent, radical change to our society, which could become similar to a hive? This book explores this reflection, which is at the center of social research on digital tools.

This book focuses on a broad range of immunomodulators, including both natural and synthetic ones. It highlights their role in human health. The book discusses nanotechnology-based immunomodulators with special emphasis on different types of formulation to boost the immune system. It highlights the role of immunomodulators against different microbes. This book describes important topics such as Classification and uses of various immunomodulators; Potential role of herbs and spices on immune system; Immune booster activity of prebiotics; Recent advances of nutraceutical and functional foods in Immune health; Antioxidants and immunomodulation; Nanotechnology and immunomodulators in cancer; Immunomodulatory role for the treatment and management of tuberculosis; Immunomodulation in autoimmune disorders; Herbal immunomodulators and COVID-19; Role of Cytokines as Immunomodulators and Immunomodulatory effects of endocrine disrupting chemicals. This book covers various aspects of product innovation, development, and launching of nutritional and pharmaceutical products with reference to immunomodulators. This book is meant for researchers and students in the field of immunology and pharmaceutical sciences.

Genomics, the mapping of the entire genetic complement of an organism, is the new frontier in biology. This handbook on the statistical issues of genomics covers current methods and the tried-and-true classical approaches.

Advances in Applied Microbiology, Volume 105 continues the comprehensive reach of this widely read and authoritative review source in microbiology. Users will find invaluable references and information on a variety of areas, with this updated volume including chapters covering The Genus Macrococcus: an insight into its evolution, biology and relationship with Staphylococcus, The use of electrobiochemical reactors for in vitro and in vivo metabolic engineering, Advances in the Microbial Ecology of Biohydrometallurgy, Optimizing yeast alcoholic fermentations, Methods to reduce spoilage and microbial contamination of plant produce, Microbial Diversity and Functional Analysis, and more.

New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Genes Biochemistry and Applications consolidates the most widely used genetic methods available, bringing together the fields of biochemistry, biotechnology, and microbiology. The chapters outlined give clear and concise direction on both standard and applied microbial genetic improvements, presenting undergraduates, postgraduates, and researchers with the latest developments in microbial gene technology. In addition, the book describes the background and usefulness of each experiment in question. All chapters covered in the book are derived from current peer-reviewed literature as accepted by the international scientific community.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Biotechnology Entrepreneurship: From Science to Solutions fills a critical gap in the biotechnology industry. For all the resources on how to start companies and on how to manage established companies in other sectors, there is a dearth of material on unique and critical issues in starting biotechnology companies, as well as managing the transition from start-up to established company. It is to this gap that Biotechnology Entrepreneurship is directed. By combining the voices of a diverse set of industry insiders with extensive experience in biotechnology, Biotechnology Entrepreneurship prepares nascent founders, managers, investors, and other biotechnology company stakeholders to position themselves and their companies for commercial success.

Several years ago, when the discovery of catalytic RNA was recognized in a public manner,many people asked if new ?elds of therapy would soon be available. Although some tentative positive answers were given,nobody would say with certainty that RNA of various kinds was a truly promising means of altering gene expression. In fact,over the past decade,both our knowledge of RNAs with different functions and the utility of RNA in the inhibition or enhancement of gene expression have occurred with great drama. We proceeded in terms of possible therapeutic tools from RNase P and group I introns through "hammerhead" RNA enzymes, antisense technology, and more recently, to RNAi and its derivatives. A useful practical method of RNA delivery in animals will complete the picture. The diversity of RNA and the varied role of it inside cells and in therapy should be a tremendous challenge for young molecular biologists. This volume will make their task easier. Sidney Altman Sterling Professor of Molecular,Cellular & Devel- mental Biology,Nobel Laureate Department of Molecular,Cellular and Developm- tal Biology Yale University V NGTPR 4/23/05 1:00 PM Page VI VI Foreword Delivery of nucleic acids to cells in an animal remains a challenging problem. It is the major obstacle to success of therapeutic approaches using genes and oli- nucleotides,including siRNAs. Solutions found so far by chemists are satisfactory only for transfection of cells in culture.

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

This volume contains the proceedings of the Eighth International Symposium on Cyclodextrins, held in Budapest, Hungary, March 31-April 2, 1996. The 147 papers collected here are milestones in the exponentially increasing cyclodextrin literature, and represent a summary of the last two years' achievement in this field, with applications in such diverse disciplines as pharmaceuticals, food, cosmetics, textiles, plastics, and chromatography. Some highlights: lipophilicity profiles of cyclodextrins by computer molecular graphics; recent toxicological studies on cyclodextrins; Buckminsterfullerene/cyclodextrin complexes; hydroxypropyl-beta-cyclodextrin; pharmacokinetics and toxicology; peracylated cyclodextrins as drug carriers; cyclodextrins in nasal drug delivery; textile fibre surface modification by a reactive cyclodextrin; cyclodextrin-containing fabric care products; drug targeting by cyclodextrin-dimers for photodynamic cancer therapy; cyclodextrins in ophthalmologic drugs; new cyclodextrin derivatives and their potentials. Audience: This book will be of interest to researchers whose work involves pharmaceuticals, food chemicals and flavours, food additives, chromatographic methods, and biotechnology, as well as fundamental cyclodextrin research.

This edited book is a comprehensive compilation of principles, conventional and molecular approaches used to develop improved varieties and hybrids of major crops in light of their origin, evolution, taxonomy, production and productivity and need by human civilization. The book covers breeding prospects of all important food and commercial crops. It highlights the importance of breeding tools and techniques in ensuring food security. This book is of interest to teachers, researchers, agriculture scientists, capacity builders, and policymakers. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture, soil science, and environmental sciences. National and international agricultural scientists and policymakers will find this book useful.

Darwin's Pangenesis and its Rediscovery Part B explores Darwin's Pangenesis, an expanded cell theory and unified theory of heredity and variation from over 150 years ago that strengthened his theory of evolution and explained many phenomena of life. Now, new discoveries on circulating DNA, mobile RNAs, prions and extracellular vesicles are providing striking evidence for the chemical existence of Darwin's imaginary gemmules. In addition, new evidence for the inheritance of acquired characters, graft hybridization, and many other phenomena that Pangenesis supposedly explains are progressing, and are hence explored in this comprehensive volume. Specific chapters in this new volume include Darwin and Mendel: The Historical Connection, Darwin's Pangenesis and Graft Hybridization, Darwin's Pangenesis and Medical Genetics, Darwin's Pangenesis and Certain Anomalous Phenomena, and Natural Selection and Pangenesis: The Darwinian Synthesis.

Fungi are an essential, fascinating and biotechnologically useful group of organisms with an incredible biotechnological potential for industrial exploitation. Knowledge of the world's fungal diversity and its use is still incomplete and fragmented. There are many opportunities to accelerate the process of filling knowledge gaps in these areas. The worldwide interest of the current era is to increase the tendency to use natural substances instead of synthetic ones. The increasing urge in society for natural ingredients has compelled biotechnologists to explore novel bioresources which can be exploited in industrial sector. Fungi, due to their unique attributes and broad range of their biological activities hold great promises for their application in biotechnology and industry. Fungi are an efficient source of antioxidants, enzymes, pigments, and many other secondary metabolites. The large scale production of fungal pigments and their utility provides natural coloration without creating harmful effects on entering the environment, a safer alternative use to synthetic colorants. The fungal enzymes can be exploited in wide range of industries such as food, detergent, paper, and also for removal toxic waste. This book will serve as valuable source of information as well as will provide new directions to researchers to conduct novel research in field of mycology. Volume 2 of "Industrially Important Fungi for Sustainable Development" provides an overview to understanding bioprospecting of fungal biomolecules and their industrial application for future sustainability. It encompasses current advanced knowledge of fungal communities and their potential biotechnological applications in industry and allied sectors. The book will be useful to scientists, researchers, and students of microbiology, biotechnology, agriculture, molecular biology, and environmental biology.

Enzymes in Food Biotechnology: Production, Applications, and Future Prospects presents a comprehensive review of enzyme research and the potential impact of enzymes on the food sector. This valuable reference brings together novel sources and technologies regarding enzymes in food production, food processing, food preservation, food engineering and food biotechnology that are useful for researchers, professionals and students. Discussions include the process of immobilization, thermal and operational stability, increased product specificity and specific activity, enzyme engineering, implementation of high-throughput techniques, screening to relatively unexplored environments, and the development of more efficient enzymes.