This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, techniques involving RCA for enhanced signal amplification, novel RCA diagnostics, sensors for expediting RCA detection, and prospective RCA-based therapeutics. This is a valuable book for university professors and students in the field of biomedical engineering and biomolecular pharmacology as well as R&D managers of biotechnology and biopharmaceutical companies. Specifically, this book: Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells Expands readers' understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads Maximizes reader insights into novel RCA diagnostics, such as PNA openers-assisted RCA for detection of single target cells and in situ RCA diagnosis of cancer cells and malignant tissues Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification Advance Praise for Rolling Circle Amplification (RCA): "This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs."- Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA) "In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts for building superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology."- Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas.

.

Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.

This represents the third volume in a series on cancer markers pub- lished by the Humana Press. The first volume, published in 1980, stressed the relationship of development and cancer as reflected in the production of markers by cancer that are also produced by normal cells during fetal development. The concept that cancer represents a problem of differentiation was introduced by Barry Pierce in describing differenti- ation of teratocarcinomas. Highlighted were lymphocyte markers, alphafetoprotein, carcinoembryonic antigen, ectopic hormones, enzymes and isozymes, pregnancy proteins, and fibronectin. The second volume, published in 1982 and coedited with Britta Wahren, focused on the diagnostic use of oncological markers in human cancers, which were systematically treated on an organ by organ basis. At that time, the application of monoclonal antibodies to the identification of cancer markers was still in a very preliminary stage. A general introduc- tion to monoclonal antibodies to human tumor antigens was given there by William Raschke, and other authors included coverage of those mark- ers then detectable by monoclonal antibodies in their chapters.

Systemic Method Mark B. Mengel, M. D. , M. P. H. Learning Objectives 3 The Biomedical Model 6 A New Framework: The Systemic Patient-Centered Method 9 Physician Roles Patient Roles 22 Patient-Centered Clinical Decision-Making Data Base Responding to Patient Cues Constructing a Mutually Agreeable Plan The Systemic Patient-Centered Method: Other Concerns Changing Larger Systems Ethics of the Systemic Patient-Centered Method Efficacy of the Systemic Patient-Centered Method 32 Safety of the Systemic Patient-Centered Method Conclusion Cases for Discussion Recommended Readings XV 36 34 32 32 31 30 30 27 25 23 16 10 Patient-Centered The 1: Chapter !iiiii!i !ili !iil !i!i !iii !iii iiii !iiiii!i !iii !iil !iii!ii! !i!i!iiiiiii !i!i !i!i!iil ii!i !i!i !iii iiii!iiiiiii !ill !i!i!iii !iii !i!i !i!ilili!iii iiii !iil i!ii iili iii! i!ii ii!i i!ii iiii iiil iiiiiii! iiii iiiiii!iiiiiiiiiii!iiii!iiii iiii iiii ii!i iiiiii!i!i!iiiiiiiii !iii iiii iiiiiiiiii!i iiiiiiiiiiii iiiiiiii iiiiiiiiiiiiii!i iiii iiii iiiiiiii iiiiiiil iii!iiii iii! iiii iiii i!i! ilil iiii iiii iiii iiii iiii iiii iiii iiiiiiii iiii!iiiiiii iiii !iiiiiii iiii iiiiii!i iiii !iii !iiii!iii!i ii!i ii!i iiil iiii !i!i!iiiii!i!ill iiiiiiii! i!i iiii iili ii!i iiiiii!i iliiii!i iili iiiiiiiiiiililil iiii iiil ilil iliiiiiiiiiiiiii ilii ilii iiii iiii iiii iiii iiii iiiiiiii ilil i!il iiil iiii ii ii iiiiiiii iiiiiiii iiii !i !i!i !i!i !i!i !i!i !i!i !i!i !i!i !i!i !ill !i!i !i!iii!iii!i !i!i !i!i !!ii !iil !iii !iii !iii !i!i iiiiiiiiiiiiiiiiiiiiii!iii!i ililili! iii iiii iiii iiiil iii iiiil iiii iiii iiii xvi Part II.

John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Leading practitioners from the University of Pennsylvania review all aspects of heart failure diagnosis and management, with a particular emphasis on office-based/ambulatory care. Following the problem-solving steps used in an office-based practice, the authors provide extensive coverage of the presenting signs and symptoms of heart failure, as well as the tools with which to evaluate left-ventricular function, hemodynamics, and exercise performance. They also discuss the complex, evidenc-based therapeutic options for treating patients with dyspnea, fatigue, or edema, following the new ACC/AHA heart failure guidelines that are specifically and directed at targeted symptoms.

The aim of this book is to help the reader achieve the correct diagnosis in the emergency setting, which continues to remain a challenge, given the variety of potential clinical presentations. Diagnostic failure is the largest reason for delays in provision of appropriate treatment, which can be life-saving, and the largest source of clinical complaints and untoward incidents leading to poor clinical outcomes and to litigation. In this book, the readers will find diagnostic checklists, organised according to potential emergency presentations and classified under body systems, including atypical presentations, lists of differential diagnoses and guidance to pattern recognition. Apart from providing an aide-memoire for a range of presentations, it is hoped that the book will allow for better quality and informed referrals, especially between health care providers. This book is aimed as a rapid reference guide for all levels of medical staff working in emergency and acute care settings in the English-speaking world, but may also benefit nursing professionals and medical students.

Classic manual muscle testing has been shown to be an invaluable clinical tool in evaluating muscle function and for the assessment of patient recovery from neuromuscular dysfunction. Advances in Professional Applied Kinesiology (PAK) have 'fine tuned' traditional understanding to bring muscle testing into another dimension. Many of the diagnostic bonds of the past have been broken, allowing for an unsurpassed range in functional diagnoses. An extremely sensitive tool, PAK muscle testing allows the clinician to evaluate a much wider array of complex systems (cranial nerve problems, joint receptor abnormalities, and acupuncture meridian imbalances) than can be made using the classic testing methods. The English language edition of this highly successful handbook has been specially prepared for the international reader. The most up-to-date PAK tests are described as well as myofascial stretch testing and post-isometric relaxation techniques. The handbook carefully explains testing procedures for almost 100 individual muscles; many more than are found in other texts on the subject. In a unique, quick reference approach, the book not only reviews muscle anatomy and physiology, but also offers many clinical pearls with regard to possible causes of muscle dysfunction. Accompanied by a wealth of illustrations, the book is clearly written in a user-friendly 'double page' design that maximizes understanding. Clearly describes modern muscle test procedures The individual aspects of each muscle are easily compared by the use of well-illustrated templated descriptions A double-page spread design allows the reader a rapid, at-a-glance access to essential information Muscle anatomy and function are succinctly recapped in order to facilitate a complete understanding of the relevance and applicability of the individual test Classic Applied Kinesiology muscle tests for detecting functional abnormalities are well described along with stretch testing and post-isometric relaxation procedures for hypertonic, shortened muscles The importance of postural analysis for the assessment of specific muscle dysfunction is discussed and plainly described Illustrates painful spondylogenic reflex points (areas) for each muscle The role of distal acupuncture points and tendinomuscular meridians in maintaining normal muscle and muscle chain function are noted in accompanying diagrams The most commonly found trigger points, their referred pain patterns and relationship to nearby acupuncture points are clearly illustrated for each muscle Perfect for use in orthopaedics, neurology, general medicine, physiotherapy, chiropractic and osteopathy

The healthcare industry is predominantly moving towards affordable, accessible, and quality health care. All organizations are striving to build communication compatibility among the wide range of devices that have operated independently. Recent developments in electronic devices have boosted the research in the medical imaging field. It incorporates several medical imaging techniques and achieves an important goal for health improvement all over the world. Despite the significant advances in high-resolution medical instruments, physicians cannot always obtain the full amount of information directly from the equipment outputs, and a large amount of data cannot be easily exploited without a computer. Machine Learning and AI Techniques in Interactive Medical Image Analysis discusses how clinical efficiency can be improved by investigating the different types of intelligent techniques and systems to get more reliable and accurate diagnostic conclusions. This book further introduces segmentation techniques to locate suspicious areas in medical images and increase the segmentation accuracy. Covering topics such as computer-aided detection, intelligent techniques, and machine learning, this premier reference source is a dynamic resource for IT specialists, computer scientists, diagnosticians, imaging specialists, medical professionals, hospital administrators, medical students, medical technicians, librarians, researchers, and academicians.

The term "electrophoresis" was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations-zone, IEF, and isotachophoresis-have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.

Use this pocket-sized guide to make nursing diagnoses and formulate care plans! Ackley and Ladwig's Guide to Nursing Diagnosis, 7th Edition provides quick access to more than 1300 specific client symptoms and a logical, step-by-step guide to care planning. A condensed version of Ackley and Ladwig's Nursing Diagnosis Handbook, this guide is updated with the 2021-2023 NANDA-I approved nursing diagnoses. Care plans are suggested for each diagnosis, including interventions, desired outcomes, and patient teaching. From nursing educators Marina Reyna Martinez-Kratz and Mary Beth Flynn Makic, this reference is ideal for use in in class, clinicals, or at the bedside! Section I: Guide to Nursing Diagnoses includes an alphabetical listing of client symptoms, client problems, medical diagnoses, psychosocial diagnoses, and clinical states, along with suggested 2021-2023 NANDA-I (R) approved nursing diagnoses, allowing you to evaluate and select an appropriate diagnosis for the client. Section II: Guide to Planning Care includes care plans for all 2021-2023 NANDA-I approved nursing diagnoses, including evidence-based, independent, collaborative, geriatric, pediatric, home care, and culturally competent interventions, as well as client/family teaching and discharge planning. Pocket-size portability makes this book easy to carry and use in in class, clinicals, or at the bedside. Alphabetical thumb tabs provide quick access to specific symptoms and nursing diagnoses. Index of NANDA-I Diagnoses is included on the inside front and back covers. NEW and UNIQUE! 2021-2023 NANDA-I (R) approved nursing diagnoses are updated with new and revised diagnoses. NEW! Updated nursing diagnoses include class and domain information consistent with the current NANDA-I.

Test-based psychological assessment has been significantly affected by the health care revolution in the United States during the past two decades. Despite new limitations on psychological services across the board and psychological testing in particular, it continues to offer a rapid and efficient method of identifying problems, planning and monitoring a course of treatment, and assessing the outcomes of interventions. This thoroughly revised and greatly expanded third edition of a classic reference, now three volumes, constitutes an invaluable resource for practitioners who in a managed care era need to focus their testing not on the general goals of personality assessment, symptom identification, and diagnosis so often presented to them as students and trainees, but on specific questions: What course of treatment should this person receive? How is it going? Was it effective? New chapters describe new tests and models and new concerns such as ethical aspects of outcomes assessment. Volume I reviews general issues and recommendations concerning the use of psychological testing for screening for psychological disturbances, planning and monitoring appropriate interventions, and the assessing outcomes, and offers specific guidelines for selecting instruments. It also considers more specific issues such as the analysis of group and individual patient data, the selection and implementation of outcomes instrumentation, and the ethics of gathering and using outcomes data. Volume II discusses psychological measures developed for use with younger children and adolescents that can be used for the purposes outlined in Volume I; Volume III, those developed for use with adults. Drawing on the knowledge and experience of a diverse group of leading experts--test developers, researchers, clinicians and others, the third edition of The Use of Psychological Testing for Treatment Planning and Outcomes Assessment provides vital assistance to all clinicians, and to their trainees and graduate students.

In the next couple of years the human genome will be fully sequenced. This will provide us with the sequence and overall function of all human genes as well as the complete genome for many micro-organisms. Subsequently it is hoped, by means of powerful bioinformatic tools, to determine the gene variants that contribute to various multifactorial diseases and genes that exist in certain infectious agents but not humans. As a consequence, this will allow us to define the most appropriate levels for drug intervention. It can be expected that the number of potential drug targets will increase, possibly by a factor of 10 or more. Nevertheless, sequencing the human genome or, for that matter, the genome of other species will only be the starting point for the understanding of their biological function. Structural genomics is a likely follow-up, combined with new techniques to validate the therapeutic relevance of such newly discovered targets. Accordingly, it can be expected that in the near future we will witness a substantial increase in novel putative targets for drugs. To address these new targets effectively, we require new approaches and innovative tools. At present, two alternative, yet complementary, techniques are employed: experimental high-throughput screening (HTS) of large compound libraries, increasingly provided by combinatorial chemistry, and computational methods for virtual screening and de novo design. As kind of status report on the maturity of virtual screening as a technique in drug design, the first workshop on new approaches in drug design and discovery was held in March 1999, at Schloss Rauischholzhausen, near Marburg in Germany. More than 80 scientists gathered and discussed their experience with the different techniques. The speakers were invited to summarize their contributions together with their impressions on the present applicability of their approach. Several of the speakers followed this request which is summarized in this publication."

A resource for practitioners who in a managed care era need to focus their testing not on the general goals of personality assessment, symptom identification, and diagnosis so often presented to them as students and trainees, but on specific questions: What course of treatment should this person receive? How is it going? Was it effective?

Like other practices, test-based psychological assessment has been significantly affected by the health care revolution in the United States during the past two decades. Despite new limitations on psychological services across the board and psychological testing in particular, it continues to offer a rapid and efficient method of identifying problems, planning and monitoring a course of treatment, and assessing the outcomes of interventions. reference, now three volumes, constitutes an invaluable resource for practitioners who in a managed care era need to focus their testing not on the general goals of personality assessment, symptom identification, and diagnosis so often presented to them as students and trainees, but on specific questions: What course of treatment should this person receive? How is it going? Was it effective? New chapters describe new tests and models and new concerns such as ethical aspects of outcomes assessment. psychological testing for screening for psychological disturbances, planning and monitoring appropriate interventions, and the assessing outcomes, and offers specific guidelines for selecting instruments. It also considers more specific issues such as the analysis of group and individual patient data, the selection and implementation of outcomes instrumentation, and the ethics of gathering and using outcomes data. Volume II discusses psychological measures developed for use with younger children and adolescents that can be used for the purposes outlined in Volume I; Volume III, those developed for use with adults. experts - test developers, researchers, clinicians and others, the third edition of The Use of Psychological Testing for Treatment Planning and Outcome Assessment provides vital assistance to all clinicians, and to their trainees and graduate students.

Recently, artificial intelligence technology has achieved much success in multiple fields, such as healthcare, security, precision agriculture, smart city, and autonomous driving. AI provides many benefits for social development, economic growth, wellbeing management, and human healthcare. Various intelligent healthcare applications have been created in order to assist in patient healthcare. The book discusses the advances of AI applications in healthcare such as disease diagnosis, diet proposal, drug prescription and trucking, and physical and psychological assistance. It also examines the applications of AI tools in healthcare such as machine learning, deep learning, soft computing, evolutionary computing techniques in the design, and implementation of healthcare solutions. This book is ideal for healthcare administrators, radiologists, medical imaging and signal specialists, diagnosticians, medical professionals, data analysts, computer science professionals, IT consultants, researchers, academicians, and students.

Antibiotics: Therapeutic Spectrum and Limitations provides up-to-date information on managing microbial infections, the development and types of antibiotics, the rationale for utilizing antibiotics, toxicity considerations, and the control of antibiotic resistance in one single resource. This book also aims to provide comprehensive insights and current trends on antibiotic therapies to treat microbial infections, their mechanisms of action, and the role of modern drug delivery in improving their efficacy. Written by leading experts from around the globe, the chapters in the book covers important aspects of microbial infections including hospital acquired infections and community acquired infections and adult sepsis, examines the various types of antibiotics with different mechanisms and therapeutic uses, the global challenge of antibiotic resistance, and clinical trials, regulatory considerations, and market overview of antibiotics. Furthermore, the chapters include updated literature reviews of the relevant key topics, high-quality illustrations, chemical structures, flowcharts, and well-organized tables, all of which enable better understanding by the readers.

Advanced Microbial Technology for Sustainable Agriculture and Environment focuses on plant-microbe interactions in respect to bioremediation and plant growth promotion, providing insights on diverse approaches such as genomics, metagenomics, proteomics, bioinformatics and other high-throughput analyses of environmentally relevant microorganisms. The impact of frequent applications of potentially toxic chemicals (pesticides and fertilizers) and increased industrialization processes on microbial diversity emphasizes the potential threat to microbial biodiversity in ecosystems. This is an ideal resource on current trends and the future of PGPR developments with bioremediation potential. Moreover, it gives a deep understanding of the genetics of microbial biodegradation and different remediation mechanisms that help to re-establish the natural environment.

Routledge is now re-issuing this prestigious series of 204 volumes originally published between 1910 and 1965. The titles include works by key figures such asC.G. Jung, Sigmund Freud, Jean Piaget, Otto Rank, James Hillman, Erich Fromm, Karen Horney and Susan Isaacs. Each volume is available on its own, as part of a themed mini-set, or as part of a specially-priced 204-volume set. A brochure listing each title in the International Library of Psychology series is available upon request.

Your essential guide in the assessment and diagnostic process. Step by step, you’ll hone your ability to perform effective health assessments, obtain valid data, interpret the findings, and recognize the range of conditions that can be indicated by specific findings to reach an accurate differential diagnosis. You’ll have coverage of 170 conditions and symptoms across the lifespan at your fingertips. Expanded, Revised & Updated! Thoroughly updated to reflect the art and the science of primary care practice as well as the newest evidence and changes in health care New Chapter!Differential Studies New & Expanded! Content in Chapter 1 on history taking-techniques and skills for special populations Expanded! Lab diagnostics information, as well as discussions of health disparities, cultural humility, and competency New Content! Genetic testing for pharmacologic prescriptions for psychiatric mental health conditions New! Diagnosis algorithm decision trees designed to help nurses assess and diagnose conditions such as chronic sore throat, chronic insomnia, and more Coverage of 170 conditions and symptoms across the life span—including children, older adults, and pregnant patients. Complaint-focused approach organized by body system, including discussions of complex conditions Step-by-step how tos for taking a focused history, performing a physical based on presenting complaints/problems, and interpreting the findings Guidance on selecting diagnostic tests and interpreting those studies to help narrow down the diagnoses Prediction rules for selected disorders Quick-reference features, including red flags ÿ assessment pearls ÿ medications causing symptoms ÿ and selected causes of symptoms

Predictive control is a powerful tool in dealing with those processes with large time delays. Generalized Predictive Control (GPC) is the most popular approach to the subject, and this text discusses the application of GPC starting with the concept of long-range predictive control and its need in medicine (particularly automated drug deliveries). The concept of adaptation is also emphasized with respect to patient-to-patient parameter variations. Subsequent chapters discuss interactions, comparisons and various aspects of GPC. The book concludes by putting into perpective the generic nature of the architecture built around GPC and which provides model-based fault diagnosis with control.

Evidence-Based Imaging presents the radiologist with a user-friendly guide to the evidence-based science and the merit behind the diagnostic imaging studies performed in medicine. This book gives the reader a clinically relevant overview of epidemiology, selection of subjects for imaging, selection of imaging strategies, imaging test performance and cost, cost-effectiveness analysis, and applicability to children. Nine major areas of medical imaging are covered, with an emphasis on common diseases. These include Oncology, Neuroimaging, Gastroenterology, Pediatrics, Respiratory System, Musculoskeletal, Cardiovascular, Trauma, and Urologic.

Radiologists, clinicians, residents, and others with an interest in medical imaging and a desire to keep current with the vast amount of evidence-based literature will find this text extremely useful.