Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
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Muscle Gene Therapy - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
Loot Price: R4,075
Discovery Miles 40 750
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Muscle Gene Therapy - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
Series: Methods in Molecular Biology, 709
Expected to ship within 10 - 15 working days
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Total price: R4,095
Discovery Miles: 40 950
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Gene therapy offers many conceptual advantages to treat muscle
diseases, especially various forms of muscular dystrophies;
however, it faces a number of unique challenges, including the need
to deliver a therapeutic vector to all muscles throughout the body.
In Muscle Gene Therapy: Methods and Protocols, expert researchers
in the field present a collection of techniques aimed at bridging
the translational gap in muscle gene therapy between the prevalent
rodent models and vitally important larger animal models. Divided
into three sections, this volume examines basic protocols for
optimizing the muscle gene expression cassette and for evaluating
the therapeutic outcomes, new developments in muscle gene therapy
technology such as adeno-associated viral vector (AAV),
oligonucleotide-mediated exon-skipping, and novel RNA-based
strategies, and step-by-step guidance on muscle gene delivery in
swine, ovine, canine, and non-human primates. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, detailed, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Muscle
Gene Therapy: Methods and Protocols serves as an invaluable
resource for graduate students, post-doctoral fellows, and
principle investigators pursuing the crucial advancement of muscle
disease gene therapy in the hope of someday curing these
debilitating disorders.
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