Scientists within the field of plant biotechnology are in a
constant search for techniques that can, in the simplest manner
possible, answer the genetic and biochemical questions that
underlie developmental processes. Thin Cell Layer Culture System
not only takes an in-depth look at a technique that has had so much
success in attempting, through various practical models and
systems, to answer these issues, but also represents a celebration
of almost 30 years of research that has covered a massive scope of
plant species and areas of study. The initial studies conducted on
tobacco thin cell layers (TCLs) - proving that organogenesis can be
strictly controlled in vitro - allowed plant research to benefit
from this finding, expanding this knowledge in a practical and
applied manner into the biotechnological fields of tissue culture
and micropropagation, cell and organ genetics and biochemistry.
The chapters in this book tell the enigmatic tale of TCLs. An
historical perspective opens the scene for an inquiry into the
possible cellular, biochemical and genetic processes that allow for
the controlled development of a TCL into any organ type. The
success of the system is further demonstrated in both
monocotyledonous and dicotyledonous species, covering successful
organogenesis and in vitro flowering in species within ornamental,
leguminous and wood crops, cereals and grasses. Methodologies are
outlined in detail, as is the rationale behind the
TCL-organogenesis-developmental sequel. The TCL method, shown to be
superior to many conventional micropropagation systems, has also
shown to be vital in the recovery of transgenic plants.
This book is an essential part of every plant, cell
anddevelopmental biologist, geneticist and tissue culturalist's
shelf as it addresses the primary issue of any plant: the cell, the
tissue, and their subsequent development into a highly organized
system.
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