Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Insoluble Proteins - Methods and Protocols (Hardcover, 2015 ed.)
Loot Price: R4,355
Discovery Miles 43 550
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Insoluble Proteins - Methods and Protocols (Hardcover, 2015 ed.)
Series: Methods in Molecular Biology, 1258
Expected to ship within 10 - 15 working days
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Total price: R4,375
Discovery Miles: 43 750
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With insolubility proving to be one of the most crippling
bottlenecks in the protein production and purification process,
this volume serves to aid researchers working in the recombinant
protein production field by describing a wide number of protocols
and examples. Insoluble Proteins: Methods and Protocols includes
chapters that describe not only the recombinant protein production
in different expression systems but also different purification and
characterization methods to finally obtain these
difficult-to-obtain proteins. Beginning with protein production
methods using both prokaryotic and eukaryotic expression systems,
the book continues with purification protocols using insoluble
proteins, the characterization of insoluble proteins, as well as a
general overview of interesting applications of insoluble proteins.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
practical, Insoluble Proteins: Methods and Protocols aims to
provide the scientific community with detailed and reliable
state-of-the-art protocols that are used in order to successfully
produce and purify recombinant proteins prone to aggregate.
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