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An Introduction to Microscopy by Means of Light, Electrons, X-Rays, or Ultrasound (Paperback, 1978 ed.)
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An Introduction to Microscopy by Means of Light, Electrons, X-Rays, or Ultrasound (Paperback, 1978 ed.)
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Many people look upon a microscope as a mere instrument(l); to them
microscopy is instrumentation. Other people consider a microscope
to be simply an aid to the eye; to them microscopy is primarily an
expan sion of macroscopy. In actuality, microscopy is both
objective and sub jective; it is seeing through an instrument by
means of the eye, and more importantly, the brain. The function of
the brain is to interpret the eye's image in terms of the object's
structure. Thought and experience are required to distinguish
structure from artifact. It is said that Galileo (1564-1642) had
his associates first look through his telescope microscope at very
familiar objects to convince them that the image was a true
representation of the object. Then he would have them proceed to
hitherto unknown worlds too far or too small to be seen with the un
aided eye. Since Galileo's time, light microscopes have been
improved so much that performance is now very close to theoretical
limits. Electron microscopes have been developed in the last four
decades to exhibit thousands of times the resolving power of the
light microscope. Through the news media everyone is made aware of
the marvelous microscopical accomplishments in imagery. However,
little or no hint is given as to what parts of the image are
derived from the specimen itself and what parts are from the
instrumentation, to say nothing of the changes made during
preparation of the specimen.
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