"Steroid Receptors: Methods and Protocols" presents a selection
of techniques that have been recently applied to the analysis of
steroid receptors, powerful tools for the advancement of our
understanding of both the mechanisms regulating gene transcription
and the rapid signaling responses of tissues to signals. Research
in this area has generated a wealth of data allowing the
elucidation of steroid receptor mechanisms and improving the
treatment of many endocrine disorders, above all cancers. Chapters
cover methods to analyze gene transcription, chromatin and
proteomic modifications, extra-nuclear signaling regulation,
development of cell and animal models, and preparation of new
antibodies. Written in the successful "Methods in Molecular
Biology" series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols and notes on
troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, "Steroid Receptors: Methods
and Protocols" offers an updated view on a variety of modern
methods that will hopefully contribute to improving our knowledge
on the integration of steroid receptors in single or different
functionally connected cellular contexts under a variety of
physiological and pathological conditions, above all tumors.
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