This second edition volume provides detailed protocols on the
theoretical background of cell cycle synchronization procedures and
instructions on how to implement these techniques. The chapters in
Cell Cycle Synchronization: Methods and Protocols, Second Edition
cover subjects such as: physical fractionations including
centrifugal elutriation of healthy and apoptotic cells, and nuclei
of mammalian cells; large scale mitotic cell synchronization;
chromosome formation during fertilization in eggs; synchronization
of unicellular organisms; hematopoietic stem cells used to improve
the engraftment in transplantation; and cell cycle control. Written
in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls. Practical and
comprehensive, Cell Cycle Synchronization: Methods and Protocols,
Second Edition is a valuable resource for PhD students and
postdoctoral fellows, and researchers interested in general
science, pharmacy, medicine and public health, computer science,
and life sciences. Specialists and professionals in cell biology,
genetics, molecular biology, biochemistry, and pharmacology will
also find this book useful.
General
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