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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Cytochrome P450 Protocols (Hardcover, 3rd ed. 2013)
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Cytochrome P450 Protocols (Hardcover, 3rd ed. 2013)
Series: Methods in Molecular Biology, 987
Expected to ship within 12 - 17 working days
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Cytochromes P450 (CYPs) comprise a large superfamily of proteins
that are of central importance in the detoxification or activation
of a tremendous number of natural and synthetic hydrophobic
xenobiotics, including many therapeutic drugs, chemical carcinogens
and environmental pollutants. CYPs are important in mediating
interactions between an organism and its chemical environment and
in the regulation of physiological processes. Cytochrome P450
Protocols, Third Edition focuses on high-throughput methods for the
simultaneous analysis of multiple CYPs, substrates or ligands.
Although the emphasis is on CYPs of mammalian origin, it reflects
an increasing interest in CYPs of bacterial species. Also included
are chapters on cytochrome P450 reductase (the redox partner of
CYPs) and the flavin-containing monooxygenases (FMOs), and
metabolomic and lipidomic approaches for identification of
endogenous substrates of CYPs ('de-orphanizing' CYP substrates).
Written in the successful Methods in Molecular Biology (TM) series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and easily accessible,
Cytochrome P450 Protocols, Third Edition provides a wide range of
techniques accessible to researchers in fields as diverse as
biochemistry, molecular biology, pharmacology, toxicology,
environmental biology and genetics.
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